Molecular detection of some virulence genes of S. aureus isolated from mastitic Cows by PCR | ||||
| Benha Veterinary Medical Journal | ||||
| Article 26, Volume 30, Issue 1, March 2016, Page 238-245 PDF (405.12 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2016.31368 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Ashraf A. Abdel-Tawab1; Fatma I. El-Hofy1; Ahmed A.A. Maarouf2; Sameh A.H. Abbas3 | ||||
| 1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University | ||||
| 2Animal Health Research Institute, Benha Branch | ||||
| 3Kaluobia Vet. Med. Organization, El-Kanater El-Khayria | ||||
| Abstract | ||||
| In this study a total of 1200 quarter milk samples were selected from 300 cows at different localities of El-Kaliobia Governorate were examined bacteriologically and revealed that 57 S.aureus isolated from both clinical and subclinical mastitis with incidence of 45.6% of total isolates. Molecular typing of S. aureus by PCR revealed that all isolates (100%) (12 random S.aureus isolates) were positive for the 16 SrRNA genes of S. aureus. Genotyping of virulence genes encoding factors of S. aureus revealed that two isolates were positive for (tst-1) gene (16.7%), three isolates positive for (etb) 25%, nine isolates positive for each of (icaD and femA) genes (75%), seven isolates positive for (hlg) (58.3%), eight isolates positive for (nuc) (66.7%). | ||||
| Keywords | ||||
| mastitis; S. aureus; PCR; Virulence genes | ||||
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