Molecular validation for impossible transmission of TYLCV through mechanical route
AbdElKareem, H., Nasr-Eldin, M., Ahmed, S., Abdelwahed, M., Soliman, E. (2023). Molecular validation for impossible transmission of TYLCV through mechanical route. EKB Journal Management System, 8(5), 185-190. doi: 10.21608/bjas.2023.198050.1107
Hager A. AbdElKareem; Mohamad A. Nasr-Eldin; Sabah A. Ahmed; Mohamed A. Abdelwahed; Elham R. S. Soliman. "Molecular validation for impossible transmission of TYLCV through mechanical route". EKB Journal Management System, 8, 5, 2023, 185-190. doi: 10.21608/bjas.2023.198050.1107
AbdElKareem, H., Nasr-Eldin, M., Ahmed, S., Abdelwahed, M., Soliman, E. (2023). 'Molecular validation for impossible transmission of TYLCV through mechanical route', EKB Journal Management System, 8(5), pp. 185-190. doi: 10.21608/bjas.2023.198050.1107
AbdElKareem, H., Nasr-Eldin, M., Ahmed, S., Abdelwahed, M., Soliman, E. Molecular validation for impossible transmission of TYLCV through mechanical route. EKB Journal Management System, 2023; 8(5): 185-190. doi: 10.21608/bjas.2023.198050.1107

Molecular validation for impossible transmission of TYLCV through mechanical route

Benha Journal of Applied Sciences
Article 16, Volume 8, Issue 5, May 2023, Page 185-190  XML PDF (332.7 K)
Document Type: Original Research Papers
DOI: 10.21608/bjas.2023.198050.1107
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Authors
Hager A. AbdElKareem email 1; Mohamad A. Nasr-Eldin2; Sabah A. Ahmed2; Mohamed A. Abdelwahed3; Elham R. S. Soliman4
1Microbiology and Botany, Science,Benha University, Benha, Egypt
2Microbiology and Botany, Science , Benha University, Benha Egypt
3Plant Genetic Transformation Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC).
4Botany and Microbiology department, Faculty of Science, Helwan University, Cairo, Egypt. Postcode: 11795
Abstract
Tomato yellow leaf curl disease (TYLCD) is a serious constraint in tomato crop worldwide, affecting the production of many crops, resulting in significant economic losses. In Egypt, the incidence of infection is very high, with visual estimates of TYLCV infection reaching 100 % in some areas. Here in the mechanical transmission ability of TYLCV is assessed in different plant species well known to be hosts for the TYLCV. The current finding revealed the failness of transmission through mechanical route. None of the mechanically infected plants developed TYLC disease symptoms. Furthermore, the absence of TYLCV genome within mechanically transmitted plants was also validated. The sequence alignment of the partial TYLCV genome amplified from the naturally infected plants from Qalyubia governorate, show 98.99% similarity and 100% query coverage to the TYLCV in the GenBank. In conclusion, the TYLCV can’t transmit through mechanical route this may be a result of failing its genome to be establish within plant genome.
Keywords
Geminivirus; Gene sequencing; Host range; Tomato
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