Prevalence and Characterization of Streptococcus pyogenes Isolates from buffalo milk, cattle milk and human milkers with M protein serotyping. | ||||
Egyptian Journal of Veterinary Sciences | ||||
Volume 54, Issue 6, November and December 2023, Page 1227-1235 PDF (1.86 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejvs.2023.212206.1513 | ||||
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Authors | ||||
Mohammed Darwish Mohammed ![]() ![]() | ||||
1microbiology department NRC | ||||
2Department of Microbiology and Immunology, Faculty of veterinary medicine, Cairo University, Cairo, Egypt. | ||||
3Department of Milk hygiene, Faculty of veterinary medicine, Cairo University, Cairo, Egypt. | ||||
4Department of Microbiology and Immunology, veterinary research institute, NRC, Cairo, Egypt. | ||||
Abstract | ||||
ABSTRACT A total of 440 raw milk samples (apparently normal) were collected from different animal farms at Sohag governorate (368from cows and 72 from bufallos) and 55 throat swap samples collected from human milkers. All the Streptococcus pyogenes (S.pyogenes) isolates were identified by their bacitracin sensitivity, positive spy1258 detection, and positive GAS latex agglutination. Different isolates were typed serotypically for M protein typing. Serotyping results indicated five different serotypes, M1, M2, M3, M4, and M6, in S.pyogenes isolates, while six isolates were identified as untypeable. Some isolates were underwent polymerase chain reaction (spy1258) for confirmation and comparison between animal and human isolated strains. In addition, antimicrobial resistance was tested to eight different antibiotics. the resistance mechanisms were determined phenotypically by the disc diffusion method. Resistance testing indicated highly resistance for mostly used antibiotics as erythromycin and clindamycin and found to be complete or inter mediate sensitive to Penicillin-G, Cefuroxime, Ampicillin /Sulbactam and Cefoperazone. | ||||
Keywords | ||||
Beta haemolytic streptococci; Group (A) streptococci; Reverse zoonosis | ||||
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