Effect of Thymoquinone on Skeletal Muscle Regeneration and Innervation in Induced Degeneration of the Hamster Buccal Pouch | ||||
| Dental Science Updates | ||||
| Article 12, Volume 4, Issue 2, September 2023, Page 313-328 PDF (3.93 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/dsu.2023.187143.1154 | ||||
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| Authors | ||||
Asmaa Mohammed Fahmy  1; Ahmed Mohammed Moheb El-Din Korraah2; Fadia Mostafa Attia3; Magda Mohammed Aly Hassan4
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| 1Oral pathology department, Faculty of Dentistry, Suez canal university | ||||
| 2Oral Pathology Department, Faculty of Dentistry, Ismailia, Suez Canal University | ||||
| 3Clinical Pathology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt | ||||
| 4Oral Pathology Department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt | ||||
| Abstract | ||||
| Introduction: Thymoquinone (TQ) has myogenic and neurogenic properties. Aim: This research aimed to study the effect of TQ on skeletal muscle regeneration and innervation in DMBA – induced degeneration of hamster buccal pouch (HBP). Material and Methods: 70 male golden Syrian hamsters were divided into 3 groups. Group A: (5) untreated animals. Group B: (5 animals), their left pouches were painted with 0.5% DMBA 3/week/6 weeks. Group C: (60 animals) was treated as group B, then divided equally into Group CI and Group CII: They received one and two i.p. TQ (0.1 mg/kg body weight) injection(s), respectively, then 5 animals from each subgroup were euthanized at 1, 2, 4, 7, 14, and 20 days. All pouches were surgically excised and processed for H&E, and immunohistochemistry stain of Pax-7 and neurofilament heavy chain (NFH). The obtained data were statistically analyzed. Results: The muscle layer’ bulk of left pouches was progressively forming from 7 and 4 days after one and two TQ injection(s), respectively. Perivascular mononuclear cells (MNCs) were Pax-7 positive from the second and first day(s) to 14 and 7 days after one and two injection(s), as well the nuclei of mature muscle fibers (MFs) from 4 and 2 days to about 20 and 14 days after one and two TQ injection(s), respectively. The intensity of NFH IHC stain was progressively increased from the second and first day(s) after one and two TQ injection(s), respectively, as MFs membranes’ plaques. Conclusion: TQ stimulated skeletal muscles’ regeneration and re-innervation in HBPs. | ||||
| Keywords | ||||
| 7,12-Dimethylbenz[a]anthracene; Muscle regeneration; Neurofilament heavy chain; Pax-7; Thymoquinone | ||||
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