ISOLATION AND IDENTIFICATION OF SALMONELLA SPP. FROM DIARRHEIC SHEEP AND GOATS IN EGYPT | ||||
| Journal of the Egyptian Veterinary Medical Association | ||||
| Volume 76, Issue 4, December 2016, Page 485-496 PDF (834.4 K) | ||||
| Document Type: Original Research Articles | ||||
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| Abstract | ||||
| Diarrhea remains one of the problems in sheep and goats causing economic losses. In the current study, a total number of 346 rectal swabs were collected at different seasons from diarrheic sheep and goats of different age groups and from different localities. Rectal swabs were bacteriologically examined for isolation of Salmonella. Five Salmonella isolates were recovered from 149 diarrheic goats and three isolates from 197 diarrheic sheep with isolation rate 3.36% and 1.52%, respectively. The isolation rate was correlated to some factors such as season, age and the localities included in the study. Where the highest seasonal rate was in Spring season followed by winter; the highest rate was in animals belonging to (1-2.5 years) age group followed by (0 up to 1 year) age group while the lowest one belongs to (>2.5 years) age group and the highest isolation rate among the localities included in the study was in El-Fayoum governorate followed by Giza governorate. All recovered isolates werebiochemically and serologically characterized and confirmed by amplification of invA gene using PCR technique. The serotyping results revealed that the 8 isolates belonged to 6 serotypes: Salmonella Bonarensis, Salmonella Paratyphi A, Salmonella Ferruch ,Salmonella Kottbus, Salmonella Stanleyville and Salmonella Enteritidis, while 2 isolates were not typed and the PCR technique confirmed that all Salmonella isolates carried the invA gene. | ||||
| Keywords | ||||
| Diarrhea; sheep; goats; Salmonella and invA | ||||
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