Molecular Identification and Characterization of Different Fusarium oxysporum f.sp. lycopersici Isolates Using RPAD-PCR Marker
Asran, M., Moharam, M., Zouhry, E. (2023). Molecular Identification and Characterization of Different Fusarium oxysporum f.sp. lycopersici Isolates Using RPAD-PCR Marker. EKB Journal Management System, 8(1), 8-19. doi: 10.21608/jsasj.2023.316166
Mahmoud R. Asran; Moustafa H. A. Moharam; Eman Zouhry. "Molecular Identification and Characterization of Different Fusarium oxysporum f.sp. lycopersici Isolates Using RPAD-PCR Marker". EKB Journal Management System, 8, 1, 2023, 8-19. doi: 10.21608/jsasj.2023.316166
Asran, M., Moharam, M., Zouhry, E. (2023). 'Molecular Identification and Characterization of Different Fusarium oxysporum f.sp. lycopersici Isolates Using RPAD-PCR Marker', EKB Journal Management System, 8(1), pp. 8-19. doi: 10.21608/jsasj.2023.316166
Asran, M., Moharam, M., Zouhry, E. Molecular Identification and Characterization of Different Fusarium oxysporum f.sp. lycopersici Isolates Using RPAD-PCR Marker. EKB Journal Management System, 2023; 8(1): 8-19. doi: 10.21608/jsasj.2023.316166

Molecular Identification and Characterization of Different Fusarium oxysporum f.sp. lycopersici Isolates Using RPAD-PCR Marker

Journal of Sohag Agriscience (JSAS)
Volume 8, Issue 1, June 2023, Page 8-19  XML PDF (1.55 MB)
Document Type: Research and Review Papers
DOI: 10.21608/jsasj.2023.316166
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Authors
Mahmoud R. Asran1; Moustafa H. A. Moharamorcid 1; Eman Zouhry email 2
1Plant Pathology Department Faculty of Agriculture Sohag University Sohag 82524 Egypt
2Plant Pathology,Faculty of Agriculture,Sohag University,82524,Sohag,Egypt.
Abstract
Five random primers of RAPD-PCR were done in eight 
isolates of Fusarium oxysporum f.sp. lycopersici, each of them 
consisted of 10 bases. In accordance with the distinction of the 
taxa based on RAPD_PCR marker analysis, the results revealed 
that, the studied isolates of were F. oxysporum f.sp. lycopersici
clearly distinguished from each other. Genetic similarity 
coefficients between pair wise varied from (0.040 to 0.429) based 
on an unweighted paired group method of arithmetic average 
(UPGMA) cluster analysis of all isolates. The dendrogram 
obtained from the data showed that, hierarchical clustering 
separated the isolates into three groups according to their 
similarity coefficients. Therefore, there were some reliable 
RAPD markers for the pathogenic isolates, which resulted close 
relationship at the molecular level under genetic control as it is 
inherited in a polygenic fashion. Thus, RAPD-PCR technique can 
be used as an important tool for the genetic identification and 
characterization of F. oxysporum f.sp. lycopersici species. 
Keywords
Fusarium; RAPD-PCR; UPGMA; dendrogram
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