Application of Spirulina platensis for developing stress tolerant and biochemical activity of micropropagated Moringa oleifera plant | ||||
| Egyptian Journal of Chemistry | ||||
| Volume 66, Issue 13, December 2023, Page 1987-1995 PDF (374.98 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2023.222707.8255 | ||||
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| Authors | ||||
Hagar Mohamed Abdel-Magied 1; Nora Muhammad Youssef 2; Eman A Ibrahim  3; Lobna Salah Taha 4; Hanaa H. Abd El Baky 5
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| 1Ornamental plants and woody trees, national research centre, Giza, Egypt. | ||||
| 2Ornamental Plants and Woody Trees Dept., National Research Centre, Dokki, Giza, Egypt. | ||||
| 3National Research Centre | ||||
| 4Ornamental plant and Woody tree Dept. Agricultural and Biological Res. Division, National Research Centre (NRC), Egypt. | ||||
| 5Plant Biochemistry Department, National Research Centre | ||||
| Abstract | ||||
| In recent years, the efforts to develop a stress tolerant of the plant are of great importance for increasing crop yield using tissue culture as a feasible and cost-effective tool for this purpose. This work aimed firstly to establish an in vitro culture of Moringa oleifera using two different types of culture media (MS and WPM) at two strengths (full or half) and three concentrations of benzyladenine (0.1, 0.2 or 0.3 mg l-1) in combination with 0.05 mg l-1 of IBA. Secondly; the Application of Spirulina platensis at 1 g l-1 as an effective stimulant on the micropropagated plants on various levels of saline media (0, 1000, 2000, 4000, and 6000 ppm of NaCl) to mitigate the effect of salinity stress. The results showed that full strength of MS culture medium supplemented with 0.3 BA mg/l + 0.05 mg/l IBA +1g charcoal /l caused the highest survived and proliferated shootlets with the highest number of roots. Alleviation of salinity stress on the in vitro grown plants at all tested levels using the S. platensis in the culture medium had noticed while, increasing salinity level to 6000 ppm caused a noticeable decline of micropropagation ability. The highest content of total flavonoid was recorded at 4000 ppm of salinity in the presence or absence of S. platensis in the culture media which also led to a decrease in the content of H2O2 radicals in shootlets. The further improvement effect of S. platensis was noticed by superoxide dismutase and glutathione peroxidase activities in shootlets grown under saline condition. | ||||
| Keywords | ||||
| Moringa oleifera; in vitro; salinity; Spirulina platensis; and superoxide dismutase activity | ||||
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