Response 5-phosphomevalonate Kinase Enzymes to Low Doses of Gamma Radiation and Its Related to the Production of Terpenoids Indole Alkaloids from Catharanthus rouses
Salama, I., Ali, G., Abd EL-Megid, M. (2019). Response 5-phosphomevalonate Kinase Enzymes to Low Doses of Gamma Radiation and Its Related to the Production of Terpenoids Indole Alkaloids from Catharanthus rouses . EKB Journal Management System, 32(1), 75-85. doi: 10.21608/ejrsa.2019.7148.1061
I. M. Salama; G. M. Ali; Mohamed H.M. Abd EL-Megid. "Response 5-phosphomevalonate Kinase Enzymes to Low Doses of Gamma Radiation and Its Related to the Production of Terpenoids Indole Alkaloids from Catharanthus rouses ". EKB Journal Management System, 32, 1, 2019, 75-85. doi: 10.21608/ejrsa.2019.7148.1061
Salama, I., Ali, G., Abd EL-Megid, M. (2019). 'Response 5-phosphomevalonate Kinase Enzymes to Low Doses of Gamma Radiation and Its Related to the Production of Terpenoids Indole Alkaloids from Catharanthus rouses ', EKB Journal Management System, 32(1), pp. 75-85. doi: 10.21608/ejrsa.2019.7148.1061
Salama, I., Ali, G., Abd EL-Megid, M. Response 5-phosphomevalonate Kinase Enzymes to Low Doses of Gamma Radiation and Its Related to the Production of Terpenoids Indole Alkaloids from Catharanthus rouses . EKB Journal Management System, 2019; 32(1): 75-85. doi: 10.21608/ejrsa.2019.7148.1061

Response 5-phosphomevalonate Kinase Enzymes to Low Doses of Gamma Radiation and Its Related to the Production of Terpenoids Indole Alkaloids from Catharanthus rouses

Egyptian Journal of Radiation Sciences and Applications
Article 7, Volume 32, Issue 1, August 2019, Page 75-85  XML PDF (998.68 K)
Document Type: Original Article
DOI: 10.21608/ejrsa.2019.7148.1061
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Authors
I. M. Salama email ; G. M. Ali; Mohamed H.M. Abd EL-Megid
Natural Products Research Department (Biotechnology Branch), National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt
Abstract
GAMMA rays effect, on different dose levels on the production of ajmalicine as well as 5-phosphomevalonate kinase enzyme activety, was studied in Catharanthus roseus cell suspension cultures. The seedlings were taken from the young leaf puds and grown on MS solid medium for 7 weeks. Calli individual serially sub cultured by transfing to maintenance medium consisting of Gamborg’s B5 medium. Cell suspensions were exposed to gamma rays after 4 weeks at dose levels of 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5 and 8Gy. Ajmalicine quantities were analyzed on VIS–spectrophotometer; also, the 5-phosphomevalonate kinase enzyme was particularly purified by ultrafiltration and activety analayzed, in addition, native PAGE electrophoresis was determined. 5-phosphomevalonate kinase enzyme bands and ajmalicine accumulation quantities were changed after the exposure to low doses from 1 to 6Gy, but the higher doses were lethal. The appropriated doses to activety of 5-phosphomevalonate kinase enzyme and Ajmalicine production were from 1 to 5Gy. Other dose levels (5.5 and 6Gy) did not affect on the 5-phosphomevalonate kinase enzyme activety and Ajmalicine production.
Keywords
Gamma rays; 5-phosphomevalonate kinase; Terpenoids indole alkaloids; <i> Catharanthus roseus </i>
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