Is Platelet Rich Plasma Effective in the Treatment of Formaldehyde Induced Arthritis in Rats? Application to Osteoarthritic Patients | ||||
| The Egyptian Journal of Anatomy | ||||
| Volume 43, Issue 1, January 2020, Page 185-208 PDF (5.25 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/EJANA.2019.19556.1048 | ||||
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| Authors | ||||
Maha Abbas Helmi Mansour1; Azza Salah El-din Soliman1; Mervat Thabet Naguib1; Enas Abd Elhafiz Mohammed1; Ahmed Farid Al Neklawy   2; Hagar Yousry Sayed Rady1
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| 1Department of Anatomy and Embryology, Faculty of Medicine, Ain Shams University, Egypt. | ||||
| 2Anatomy and Embryology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt., Department of Physiological Sciences, Fakeeh College for Medical Sciences, Jeddah, Kingdom of Saudi Arabia. | ||||
| Abstract | ||||
| Introduction: In this study, the efficacy of platelet rich plasma (PRP) in the treatment of formaldehyde (FA) induced arthritis was evaluated. Material and Methods: Forty two adult male albino rats were divided into three groups. Group I (control group): eighteen rats were divided into I-a, I-b, and I-c subgroups. Group II (arthritic group): twelve rats were subjected to intra-articular injection with single dose of 0.02 ml of 5% FA in the right knee and were further subdivided into II-a and II-b subgroups. Group III (arthritic group treated with PRP): twelve rats were subjected to induced arthritis as in group II. Then they were subjected to intra-articular injection with single dose of 0.3 ml of PRP in the same right knee and were further subdivided into III-a and III-b subgroups. Results: After sacrifice, the knee joints were fixed, decalcified, and processed for paraffin sections. Different stains were applied and immunohistochemical staining for Caspase-3 enzyme was done. Sections were examined by light microscope. The arthritic groups revealed irregular articular surface and bone eburnation. There was apparent hypocellularity and disorganization of the chondrocytes. Osteoclasts and osteoblasts invaded the osteochondral junction. The synovial membrane showed deposition of thick collagen bundles with inflammatory cell infiltrate and numerous blood vessels. Caspase-3 immunoreactivity was apparent in many chondrocytes. All these changes were ameliorated in group III-a. Many of these changes were seen in Group III-b. The morphometric results and statistical analysis confirmed the histological findings. Conclusion: It was concluded that intra-articular injection of PRP demonstrated transient effective role on articular cartilage healing. | ||||
| Keywords | ||||
| Articular cartilage; efficacy of platelet rich plasma; formaldehyde; induced arthritis; platelet rich plasma | ||||
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