Eco-Friendly Green Synthesis of Silver Nanoparticles from Egyptian Honey: Evaluating its Antibacterial Activities | ||||
| Egyptian Journal of Botany | ||||
| Article 13, Volume 59, Issue 3, November 2019, Page 709-721 PDF (3.05 MB) | ||||
| Document Type: Regular issue (Original Article) | ||||
| DOI: 10.21608/ejbo.2019.6597.1261 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ghada A. Youssef  ; Aliaa M. El-Boraey; Mai M. Abdel-Tawab
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| Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt | ||||
| Abstract | ||||
| THIS RESEARCH article describes a strong approach for the green synthesis of silver nanoparticles (AgNPs) that employs a local black seed honey (BSH). Honey was chosen as the eco-friendly reducing and stabilizing agent replacing most reported reducing agents which represent highly biological risk to the society and environment. Honey reduced silver ions into AgNPs after 20min in a stirred reaction mixture. Nanoparticles of different sizes were obtained, and the solution turned black over time. The antibacterial activity of AgNPs was investigated against eight pathogenic bacterial strains; five Gram-negative and three Gram-positive. AgNPs were potentially effective against the Gram-negative more than Gram-positive bacterial strains. E. coli and P. aeruginosa were the highly susceptible strains with 6.3μg/ml minimum inhibitory concentrations (MICs). The AgNPs were characterized by perform UV-visible spectroscopy, fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and scanning electron microscopy (SEM) imaging. The colloid obtained at a pH of 9 was found to be spherical in shape and was distributed with a certain amount of agglomeration. An intense surface plasmon resonance band at 400nm in the UV-visible spectrum clearly revealed the formation of AgNPs after 72hr. TEM showed spherically shaped AgNPs, and the sizes of the nanoparticles ranged from 25-70nm. AgNPs had broad spectrum antibacterial property thus it can be explored further for medical and therapeutic applications. | ||||
| Keywords | ||||
| Black seed honey (BSH); Green synthesis; Characterization of silver nanoparticles; Pathogenic strains; Antibacterial activity | ||||
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