THE siRNA EFFICACY OF SOLUBLE ACID INVERTASE DOWN-REGULATION IN SUGARCANE (SACCHARUM SPP.)
Khaled, S., Abdel-Tawab, F., Fahmy, E., Amer, E., Khaled, K. (2018). THE siRNA EFFICACY OF SOLUBLE ACID INVERTASE DOWN-REGULATION IN SUGARCANE (SACCHARUM SPP.). EKB Journal Management System, 26(Special issue (2C)), 2011-2017. doi: 10.21608/ajs.2018.31668
Shereen K.M. Khaled; F.M. Abdel-Tawab; Eman M. Fahmy; E.A.M. Amer; K.A. Khaled. "THE siRNA EFFICACY OF SOLUBLE ACID INVERTASE DOWN-REGULATION IN SUGARCANE (SACCHARUM SPP.)". EKB Journal Management System, 26, Special issue (2C), 2018, 2011-2017. doi: 10.21608/ajs.2018.31668
Khaled, S., Abdel-Tawab, F., Fahmy, E., Amer, E., Khaled, K. (2018). 'THE siRNA EFFICACY OF SOLUBLE ACID INVERTASE DOWN-REGULATION IN SUGARCANE (SACCHARUM SPP.)', EKB Journal Management System, 26(Special issue (2C)), pp. 2011-2017. doi: 10.21608/ajs.2018.31668
Khaled, S., Abdel-Tawab, F., Fahmy, E., Amer, E., Khaled, K. THE siRNA EFFICACY OF SOLUBLE ACID INVERTASE DOWN-REGULATION IN SUGARCANE (SACCHARUM SPP.). EKB Journal Management System, 2018; 26(Special issue (2C)): 2011-2017. doi: 10.21608/ajs.2018.31668

THE siRNA EFFICACY OF SOLUBLE ACID INVERTASE DOWN-REGULATION IN SUGARCANE (SACCHARUM SPP.)

Arab Universities Journal of Agricultural Sciences
Article 26, Volume 26, Special issue (2C), May 2018, Page 2011-2017  XML PDF (697.34 K)
Document Type: Original Article
DOI: 10.21608/ajs.2018.31668
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Authors
Shereen K.M. Khaled1; F.M. Abdel-Tawab2; Eman M. Fahmy2; E.A.M. Amer1; K.A. Khaled3
1Sugar Crop Research Institue (SCRI), Agriculture Research Center (ARC), Giza, Egypt
2Genetics Dept., Fac. of Agric., Ain-Shams Univ., P.O. Box 68 Hadayek Shoubra 11241, Cairo, Egypt
3Genetics Dept., Fac. of Agric., Beni-suef Univ., Beni-suef, Egypt
Abstract
Sugarcane (Saccharum sp. hybrids) is a C4 grass used as a major source of sucrose. Invertase enzymes hydrolyse sucrose into hexose sugars reducing the production markedly. Soluble acid invertase role is always a case of discussion for having a major or minor role in the breakdown process in sink tissues. Bio-deterioration is another serious problem accomplishes the sucrose production, the delay between harvest and milling of sugarcane cause enormous depreciation in cane tonnage as well as sugar recovery. Beside another many factors, it was improved that both neutral and acid invertase present in cane stalk and both have tendency to increase after harvest. In the present study, sugarcane cultivar G.99/103, Saccharum
 officinarium, was used to establish Inplanta transformation experiment for down-regulation of soluble acid invertase gene using siRNA application. The transgenic plants were examined chemically and genetically to estimate the percentage of silencing and its impact on the sucrose content. The enzyme activity showed reduction compared to control in most transgenic plants and consequently the decrease in expression level of soluble acid invertase increase the Brix value significantly in some of the transgenic plants. 
 
Keywords
Sugarcane; In-Planta transformation; SAI; siRNA; RT-PCR; enzyme activity; brix
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