Molecular characterization of some fungi isolated from broiler chicken farms
Abd El Tawab, A., Maarouf, A., El-Hofy, F., Ahmed, K. (2015). Molecular characterization of some fungi isolated from broiler chicken farms. EKB Journal Management System, 29(2), 106-118. doi: 10.21608/bvmj.2015.31682
Ashraf A. Abd El Tawab; Ahmed A. A. Maarouf; Fatma I. El-Hofy; Khalid, S.M. Ahmed. "Molecular characterization of some fungi isolated from broiler chicken farms". EKB Journal Management System, 29, 2, 2015, 106-118. doi: 10.21608/bvmj.2015.31682
Abd El Tawab, A., Maarouf, A., El-Hofy, F., Ahmed, K. (2015). 'Molecular characterization of some fungi isolated from broiler chicken farms', EKB Journal Management System, 29(2), pp. 106-118. doi: 10.21608/bvmj.2015.31682
Abd El Tawab, A., Maarouf, A., El-Hofy, F., Ahmed, K. Molecular characterization of some fungi isolated from broiler chicken farms. EKB Journal Management System, 2015; 29(2): 106-118. doi: 10.21608/bvmj.2015.31682

Molecular characterization of some fungi isolated from broiler chicken farms

Benha Veterinary Medical Journal
Article 13, Volume 29, Issue 2, December 2015, Page 106-118  XML PDF (376.03 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2015.31682
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Authors
Ashraf A. Abd El Tawab1; Ahmed A. A. Maarouf2; Fatma I. El-Hofy1; Khalid, S.M. Ahmed3
1Bacteriology, Immunology and Mycology Dep., Fac. Vet. Med. Benha Univ.
2Animal Health Research "Benhabranch"
3Animal Health Research "Mansoura branch"
Abstract
The study was performed on 225 chicken (72 diseased and 153 freshly dead ones) from different 45
commercial broiler farms (1- 30 days old) at Dakahlia Governorate for mycotic infection. Samples were
taken from these chickens (lung; air sac; crop; liver and brain from each chicken) after clinical and
postmortem examination for mycological examination. The results revealed that, fungi were isolated
from 503 positive samples (44.7%); represented as 119 positive samples (10.6%) from diseased chickens
and 384 positive samples (34.1 %) from freshly dead ones.651 fungal isolates (moulds and yeasts) were
isolated from 1125 samples, where 219(33.6%) were isolated from lung samples followed by
199(30.6%); 119(18.3%); 91(14.0%) and 23(3.5%) from air sac, liver, crop and brain samples
respectively. Moreover, A. fumigatus was the most isolated one 141(21.7%) followed by A. flavus
126(19.4%); A. niger 111(17.1%); Rhizopus spp. 67(10.3%); C. albicans 58(8.9%); Mucor spp.
42(6.5%); Penicillium spp. 32(4.9%); A. terreus 20(3.1%); Fusarium spp.19 (2.9%); C. krusei 13(2.0%);
C. tropicalis 9(1.4%); A. candidus 9(1.4%) and finally A. ochraceus 4(0.6%). PCR using fungus-specific
universal primer pairs (ITS1 and ITS4) was used for identification and genotypic characters of
Aspergillus species (A. fumigatus; A. flavus and A. niger).They were able to successfully amplify the
ITS1–5.8S rDNA region of all tested Aspergillus isolates, providing a single PCR product of about 600
bp for all tested isolates PCR using fungus-specific universal primer pairs (ITS and RPS) was used for
identification and genotypic characters of C. providing a single PCR product of about 109 bp .Sequence
of the internal transcribed spacer 1 reigon (ITS-1) of isolated A. flavus Gene Bank accession number
for studied nucleotide sequence (Bankit 1867303) is KM 983253. The sequences obtained for ITS-1
region were more than 98% identical to the corresponding GenBank sequences (accession no.
DQ467968.1; KC994648.1; LN482516.1 and KP689246.1). Sequence of the internal transcribed spacer
1 region (ITS-1) of isolated C. albicans. Gene Bank accession number for studied nucleotide sequence
(Bankit 1867955) is KM 983254. The sequences obtained for ITS-1 region obtained were more than
95% identical to the corresponding GenBank (accession no. M87288.1; gb|S71769.1|S71769; emb
(AL033396.1) and AP006852.1).
Keywords
C. albicans; A. flavus; A. ochraceus
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