Detection of FMD virus antigens and antibodies using staph protein-a rapid agglutination test in comparison with the used traditional methods
Farouk, E., H., K., Abd Elazeiz, H. (2015). Detection of FMD virus antigens and antibodies using staph protein-a rapid agglutination test in comparison with the used traditional methods. EKB Journal Management System, 29(2), 129-135. doi: 10.21608/bvmj.2015.31684
Ekbal M. Farouk; Khodeir M. H.; Hanaa Abd Elazeiz. "Detection of FMD virus antigens and antibodies using staph protein-a rapid agglutination test in comparison with the used traditional methods". EKB Journal Management System, 29, 2, 2015, 129-135. doi: 10.21608/bvmj.2015.31684
Farouk, E., H., K., Abd Elazeiz, H. (2015). 'Detection of FMD virus antigens and antibodies using staph protein-a rapid agglutination test in comparison with the used traditional methods', EKB Journal Management System, 29(2), pp. 129-135. doi: 10.21608/bvmj.2015.31684
Farouk, E., H., K., Abd Elazeiz, H. Detection of FMD virus antigens and antibodies using staph protein-a rapid agglutination test in comparison with the used traditional methods. EKB Journal Management System, 2015; 29(2): 129-135. doi: 10.21608/bvmj.2015.31684

Detection of FMD virus antigens and antibodies using staph protein-a rapid agglutination test in comparison with the used traditional methods

Benha Veterinary Medical Journal
Article 15, Volume 29, Issue 2, December 2015, Page 129-135  XML PDF (251.97 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2015.31684
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Authors
Ekbal M. Farouk1; Khodeir M. H.1; Hanaa Abd Elazeiz2
1Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo
2Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo
Abstract
Considering rapid accurate detection of FMD virus antigens and antibodies is an essential corner stone
in the epidemiological studies; Staphylococcal protein-A (SPA) was successfully prepared and used for
application of rapid agglutination test (RAT) on both of processed infected tongue epithelium and
infected cell culture fluid for detection of FMD virus antigens using reference viruses and anti-sera. It
was found that the traditional virus neutralization test (VNT) consuming long time (18-24 hours) and
RAT with the shortest time (2-5 minutes) were able to detect the antigens of FMDV type O; A and SAT2
with titer ranged from ≤ log10 up to 7log10 TCID50/ml. In addition, RAT carried on serum samples
obtained from vaccinated animals revealed positive results with titers similar to those obtained by serum
neutralization test (SNT) ranged from ≤ 2 up to 256 for the three virus types with shorter time (2-5
minutes) than that consumed by SNT (18-24 hours). It was noticed that high antigen or antibody titers
showed strong agglutination while weak agglutination was noticed with lower titers. So it could be
concluded that the present work provides a rapid; sensitive and accurate test to detect FMDV antigens
and antibodies as safe; easy to be used, highly sensitive, rapid field test, of low cost and having friendly
environmental relationship.
Keywords
Foot and mouth disease (FMD); Staph protein A (SPA); Rapid agglutination test (RAT)
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