Evaluation of the therapeutic effect of Zingiber Officinale loaded on nanoparticles for cryptosporidiosis in experimentally immunosuppressed mice | ||||
| Parasitologists United Journal | ||||
| Article 7, Volume 16, Issue 2, August 2023, Page 133-138 PDF (834.75 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/PUJ.2023.209631.1211 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Hagar Abdelmaksoud   1; Engy Nahas2; Mousa Ismail3; Enas Abu-Sarea4; Hala El-Askary 5; Rabab Zalat6; Shaimaa Elgohary7; Shimaa Ibrahim8
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| 1Departments of Parasitology Cairo ,Egypt | ||||
| 2Departments of Parasitology Faculties of Medicine,Beni Suef, Beni Suef, Egypt | ||||
| 3Departments of Parasitology, Faculties of Medicine,Cairo Cairo Egypt | ||||
| 4Departments of Parasitology, Faculties of Medicine. Beni Suef Universities, Beni Suef , | ||||
| 5Departments of Parasitology, Faculties of Medicine, Beni Suef, Universities, Beni Suef | ||||
| 6Departments of Parasitology, Theodor Bilharz Research Institute , Cairo | ||||
| 7Departments of Parasitology Pathology Faculties of Medicine, Ain Shams , Cairo, Egypt | ||||
| 8Departments of Parasitology, Faculties of Medicine, Beni Suef Universities, Beni Suef | ||||
| Abstract | ||||
| Background: Cryptosporidiosis causes retractable diarrhea with drastic dehydration, especially in immunocompromised patients. Search for a novel therapeutic approach is urgently required. Objective: To assess the potential effect of Zingiber Officinale (ginger) loaded on chitosan nanoparticles (ginger CSNPs) versus Nanazoxid® (NTZ) in treatment of cryptosporidiosis. Material and Methods: Seventy mice were immunosuppressed and divided into seven groups (10 mice each), of which two groups were negative and positive control groups. Except for the negative control group, cryptosporidiosis was experimentally induced, and different drug regimens (NTZ, ginger, CSNPs, ginger CSNPs, and NTZ combined with ginger CSNPs) were given for seven consecutive days. Stool samples were collected daily for oocysts count, followed by sacrifice of mice one week after drug administration. Histopathological examination was conducted for small intestine, liver, and lung tissues. Results: Treatment with ginger CSNPs recorded the highest significant reduction in oocysts shedding, followed by treatment with NTZ/ginger CSNPs combined therapy. Histopathological examination of small intestine showed the best amelioration results in mice treated with ginger CSNPs signified by remarkable improvement in the form of intact intestinal mucosa, with complete healing of intestinal surface epithelium, and with preserved brush border and absence of mucosal ulceration. Histopathologic effect in liver showed various degrees of expansion of portal tracts with moderate inflammation among the treated groups. The lung showed congestion with various degrees of emphysematous changes and inflammatory infiltration among the treated groups. Conclusion: Ginger CSNPs possessed anti-cryptosporidial activity, diminished the oocysts shedding, and protected the intestinal epithelial from deleterious effects of cryptosporidiosis. | ||||
| Keywords | ||||
| chitosan; cryptosporidiosis; ginger; histopathology; immunosuppressed; in vivo; nanoparticles | ||||
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