Evaluation of oral immunization of sheep with Brucella Melitensis vaccine REV.1 in combination with Flagellin against a virulent Brucella melitensis 16 M strain
Farahat, L., El-Ayouby, S., Abdelwahab, M., Ebied, M., Moustafa, A. (2015). Evaluation of oral immunization of sheep with Brucella Melitensis vaccine REV.1 in combination with Flagellin against a virulent Brucella melitensis 16 M strain. EKB Journal Management System, 29(2), 200-207. doi: 10.21608/bvmj.2015.31709
Lubna F. Farahat; Saleh M.S. El-Ayouby; Mohamed G. Abdelwahab; Mohamed H. Ebied; Abdel-Moneim Moustafa. "Evaluation of oral immunization of sheep with Brucella Melitensis vaccine REV.1 in combination with Flagellin against a virulent Brucella melitensis 16 M strain". EKB Journal Management System, 29, 2, 2015, 200-207. doi: 10.21608/bvmj.2015.31709
Farahat, L., El-Ayouby, S., Abdelwahab, M., Ebied, M., Moustafa, A. (2015). 'Evaluation of oral immunization of sheep with Brucella Melitensis vaccine REV.1 in combination with Flagellin against a virulent Brucella melitensis 16 M strain', EKB Journal Management System, 29(2), pp. 200-207. doi: 10.21608/bvmj.2015.31709
Farahat, L., El-Ayouby, S., Abdelwahab, M., Ebied, M., Moustafa, A. Evaluation of oral immunization of sheep with Brucella Melitensis vaccine REV.1 in combination with Flagellin against a virulent Brucella melitensis 16 M strain. EKB Journal Management System, 2015; 29(2): 200-207. doi: 10.21608/bvmj.2015.31709

Evaluation of oral immunization of sheep with Brucella Melitensis vaccine REV.1 in combination with Flagellin against a virulent Brucella melitensis 16 M strain

Benha Veterinary Medical Journal
Article 24, Volume 29, Issue 2, December 2015, Page 200-207  XML PDF (300.52 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2015.31709
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Authors
Lubna F. Farahat1; Saleh M.S. El-Ayouby1; Mohamed G. Abdelwahab2; Mohamed H. Ebied2; Abdel-Moneim Moustafa2
1Antigens and Serums Dep., VSVRI. Abbasia, Cairo Egypt
2Animal Medicine Department Faculty of veterinary Medicine Benha University, Egypt
Abstract
In this work five vaccination protocols were prepared and applied these protocols in sheep. Serum
samples were collected from each group at time points 25, 50, 77, 89, and 98 days post vaccination. The
results revealed that humoral antibodies were detected in groups 3, 4 and 5 by using Rose Bengal test
(RBT), Buffer Acidified Plate Antigen Test (BAPAT), and Tube Agglutination Test at 98 days, 77 days,
and 77 days respectively, while for ELISA test revealed that were positive at 50, 89, 98, 98, and 98 days
post vaccination in group 1, 2,3,4 and 5 respectively. Cell mediated immunity was evaluated by
Lymphocyte Blastogenesis Assay Test and Brucellin test (Delayed Type Hyper Sensitivity Test). The
results indicated that there were no significant differences in between mean of different groups at P ≤
0.05, So for Skin Delayed Hyper Sensitivity test, Group 1 and group 6 were negative while Group 2, 3,
4 and 5 were positive. Conclusion, animals in Groups 3, 4 and 5 had humoral immune response and can
be protected from abortion in pregnant ewes and prevent infection. In this work, we evaluated to
potential of three doses reduced Rev.1 mixed with E. coli flagellin which induced protection without
need of adjuvant against I/P Brucella melitensis challenge. Also these data suggest that flagellin proteins
might induce protective immune responses and these proteins will be a good candidate for subunit
vaccine against ovine brucellosis in sheep.
Keywords
Brucella melitensis; Rose Bengal Test; Buffer Acidified Plate Antigen Test
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