Impairment of Insulin Signalling Pathway: AMPKα/SIRT1 and Pathophysiological Involvement of Gut Microbiota in Monosodium Glutamate Induced Polycystic Ovary Syndrome with Palliative Role of Flaxseed.
Awad, M., Ayad, N., Ibrahim, S., Azzam, A., Helal, B., El-shaer, R. (2023). Impairment of Insulin Signalling Pathway: AMPKα/SIRT1 and Pathophysiological Involvement of Gut Microbiota in Monosodium Glutamate Induced Polycystic Ovary Syndrome with Palliative Role of Flaxseed.. EKB Journal Management System, 43(4), 278-305. doi: 10.21608/besps.2023.108924.1149
Marwa Mahmoud Awad; Nada Hashem Mohamed Ayad; Sarah Ibrahim; Asmaa Azzam; Basma Abdel Fattah Helal; Rehab Ahmed Ahmed El-shaer. "Impairment of Insulin Signalling Pathway: AMPKα/SIRT1 and Pathophysiological Involvement of Gut Microbiota in Monosodium Glutamate Induced Polycystic Ovary Syndrome with Palliative Role of Flaxseed.". EKB Journal Management System, 43, 4, 2023, 278-305. doi: 10.21608/besps.2023.108924.1149
Awad, M., Ayad, N., Ibrahim, S., Azzam, A., Helal, B., El-shaer, R. (2023). 'Impairment of Insulin Signalling Pathway: AMPKα/SIRT1 and Pathophysiological Involvement of Gut Microbiota in Monosodium Glutamate Induced Polycystic Ovary Syndrome with Palliative Role of Flaxseed.', EKB Journal Management System, 43(4), pp. 278-305. doi: 10.21608/besps.2023.108924.1149
Awad, M., Ayad, N., Ibrahim, S., Azzam, A., Helal, B., El-shaer, R. Impairment of Insulin Signalling Pathway: AMPKα/SIRT1 and Pathophysiological Involvement of Gut Microbiota in Monosodium Glutamate Induced Polycystic Ovary Syndrome with Palliative Role of Flaxseed.. EKB Journal Management System, 2023; 43(4): 278-305. doi: 10.21608/besps.2023.108924.1149

Impairment of Insulin Signalling Pathway: AMPKα/SIRT1 and Pathophysiological Involvement of Gut Microbiota in Monosodium Glutamate Induced Polycystic Ovary Syndrome with Palliative Role of Flaxseed.

Bulletin of Egyptian Society for Physiological Sciences
Volume 43, Issue 4, October 2023, Page 278-305  XML PDF (2.66 MB)
Document Type: Original Article
DOI: 10.21608/besps.2023.108924.1149
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Authors
Marwa Mahmoud Awad email orcid 1; Nada Hashem Mohamed Ayadorcid 2; Sarah Ibrahimorcid 3; Asmaa Azzamorcid 3; Basma Abdel Fattah Helalorcid 1; Rehab Ahmed Ahmed El-shaerorcid 1
1Physiology Department, Faculty of Medicine,Tanta University, Tanta, Egypt
2Biochemistry Department, Faculty of Medicine, Tanta University, Tanta , Egypt
3Anatomy and Embryology Department, Faculty of Medicine, Tanta University, Tanta, Egypt
Abstract
Aims: This study aims to investigate the role of gut microbiota in polycystic ovarian syndrome (PCOS) induction by monosodium glutamate (MSG) with the study of the protective effect of flaxseeds. Methods: 24 female rats were divided into three groups: Group 1: distilled water; Group 2: MSG; and Group 3: flaxseed + MSG. Finally, body and ovarian weights, HOMA-IR, plasma (gonadal hormones, triglycerides, HDL, LDL, adiponectin, TNF-α, IL-6, and lipopolysaccharides), intestinal (alkaline phosphatase, myeloperoxidase, Trimethylamine N-oxide, and free fatty acid receptor 2 gene expression), ovarian tissue (Silent Information Regulator 1 (SIRT1), reduced glutathione, and gene expression of mitogen activated protein kinase (AMPKα) and SIRT1) were determined. In addition, vaginal swaps, ovarian histopathology, and immunohistochemical staining of BCL2 and Bax were done. Results: MSG-induced insulin resistance, dyslipidemia, intestinal dysbiosis, ovarian oxidative stress, and inflammation through modulation of the AMPKα/SIRT1 pathway. On the other hand, flaxseed significantly prevented these findings. Conclusion: MSG-induced gut dysbiosis that predisposes to PCOS and ingestion of flaxseed can be considered a prospective protective agent against PCOS development.
Keywords
PCO; MSG; Gut mictrobiota
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