Detection and pathotyping of a recent Newcastle disease virus outbreak in Egypt | ||||
| Benha Veterinary Medical Journal | ||||
| Article 36, Volume 29, Issue 2, December 2015, Page 297-302 PDF (265.96 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2015.31739 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Gabr F. El-Bagoury1; Ehab M. El-Nahas1; Mahmoud M. Abd-El-Monem2; Mohamed A. AboElkhair2 | ||||
| 1Department of virology, Faculty of Veterinary Medicine, Benha University | ||||
| 2Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo | ||||
| Abstract | ||||
| This study was conducted to detect and pathotype Newcastle disease virus (NDV) isolates from a recent outbreaks affecting poultry farms in Egypt between 2013 and 2014. Trachea, liver and brains were collected from clinically infected NDV three different vaccinated broiler farms in Sharqia Province. The reverse transcription polymerase chain reaction (RT-PCR) using fusion (F) gene specific primers were more sensitive than haemagglutination (HA) in the detection of NDV in collected samples. Inoculation of all the collected samples in 10-day-old embryonated chicken specific-pathogen-free eggs resulted in isolation of haemagglutinating agents in 18 samples. These haemagglutinating agents were confirmed as NDV by haemagglutination inhibition (HI) test. The intracerebral pathogenicity index (ICPI) revealed that all isolates have a range >1.5 index which congruent to velogenic type. These results confirmed that the circulating NDV strains are virulent for chickens and vaccination failures occur | ||||
| Keywords | ||||
| NDV; chicken; ICPI; RT-PCR | ||||
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