Efficacy of a locally prepared bovine mastitis vaccine

L., Sayed M.; S., Shell W.; Ahmed, Hanan A.; Ibrahim, Hanan M.; A., Nasr E.; M., Ali A.

doi:10.21608/bvmj.2015.31741

	Efficacy of a locally prepared bovine mastitis vaccine
Benha Veterinary Medical Journal
Article 38, Volume 29, Issue 2, December 2015, Page 309-318 PDF (777.74 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2015.31741
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Authors
Sayed M. L.¹; Shell W. S.¹; Hanan A. Ahmed¹; Hanan M. Ibrahim¹; Nasr E. A.²; Ali A. M.²
¹Central Laboratory for Evaluation of Veterinary Biologics
²Veterinary Serum and Vaccine Research Institute. Abassia, Cairo, Egypt
Abstract
The objective of the present work is to prepare and evaluate the effectiveness of a locally prepared polyvalent vaccine against mastitis from the most common causes of mastitis. Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli were the most prevalent bacteria recovered from clinical and subclinical mastitis. Montanide ISA-206 adjuvanted inactivated polyvalent vaccine containing the three strains was prepared. Twenty pregnant cows were inoculated intramuscularly with the prepared polyvalent vaccine two months prior to calving and boostering at day 21 from the primary injection. Serum samples from vaccinated and non-vaccinated cows were collected at the 1^st, 3^rd, 8^th, 12^th, 16^th, 20^th and 24^th weeks post vaccination and evaluated immunologically using ELISA. The results showed that immune response was significantly higher in the vaccinated group than that of controls. These results could be indicated the safety and effectiveness of the vaccine in reduction of incidence and severity of clinical cases of mastitis but further studies should be done to elucidate the possibility of field application and effectively.
Keywords
mastitis; vaccine; Staphylococcus aureus; Streptococcus agalactiae; Escherichia coli


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