Genetic Variation among Five Egyptian Clover Cultivars Using Random Amplified Polymorphic DNA (RAPD) and Sequence-Related Amplified Polymorphism (SRAP) Molecular Markers
Youssef, S., Ibrahim, S., Abdel Azim, A., Khashaba, E., El-Sharkawy, A. (2019). Genetic Variation among Five Egyptian Clover Cultivars Using Random Amplified Polymorphic DNA (RAPD) and Sequence-Related Amplified Polymorphism (SRAP) Molecular Markers. EKB Journal Management System, 11(2), 37-45. doi: 10.21608/eajbsc.2019.31758
Sawsan S. Youssef; Sanaa A. Ibrahim; Amany M. Abdel Azim; Etr H. K. Khashaba; Ahmed M. El-Sharkawy. "Genetic Variation among Five Egyptian Clover Cultivars Using Random Amplified Polymorphic DNA (RAPD) and Sequence-Related Amplified Polymorphism (SRAP) Molecular Markers". EKB Journal Management System, 11, 2, 2019, 37-45. doi: 10.21608/eajbsc.2019.31758
Youssef, S., Ibrahim, S., Abdel Azim, A., Khashaba, E., El-Sharkawy, A. (2019). 'Genetic Variation among Five Egyptian Clover Cultivars Using Random Amplified Polymorphic DNA (RAPD) and Sequence-Related Amplified Polymorphism (SRAP) Molecular Markers', EKB Journal Management System, 11(2), pp. 37-45. doi: 10.21608/eajbsc.2019.31758
Youssef, S., Ibrahim, S., Abdel Azim, A., Khashaba, E., El-Sharkawy, A. Genetic Variation among Five Egyptian Clover Cultivars Using Random Amplified Polymorphic DNA (RAPD) and Sequence-Related Amplified Polymorphism (SRAP) Molecular Markers. EKB Journal Management System, 2019; 11(2): 37-45. doi: 10.21608/eajbsc.2019.31758

Genetic Variation among Five Egyptian Clover Cultivars Using Random Amplified Polymorphic DNA (RAPD) and Sequence-Related Amplified Polymorphism (SRAP) Molecular Markers

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Article 4, Volume 11, Issue 2, March 2019, Page 37-45  XML PDF (1.13 MB)
Document Type: Original Article
DOI: 10.21608/eajbsc.2019.31758
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Authors
Sawsan S. Youssef1; Sanaa A. Ibrahim2; Amany M. Abdel Azim2; Etr H. K. Khashaba2; Ahmed M. El-Sharkawy1
1Department of Genetics, Faculty of Agriculture Cairo University, 12613, Giza- Egypt
2Plant Protection Research Institute, Agriculture Research Center, Dokki, Egypt.
Abstract
To estimate the genetic variation among five Egyptian clover cultivars, Random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) analyses were employed. Sixteen RAPD and six SRAP primers were used in the present study, a total of 34 and 12 bands showed 24.8 and 60% polymorphism percentage. The highest number of RAPD bands was recorded for primers OPE-B-07 (13 bands), followed by OPE-K-04 (12 bands), while the lowest was scored for OPE-B-05 (5 bands). For the SRAP Primers Me1-em2, Me3-em2 and Me3-em4 recorded the highest number of bands (four bands), followed by three bands for Me1-em1and Me3-em3, primer Me3-em1 recorded the lowest band number (two bands only). The dendrogram built based on combined data from RAPD and SRAP analyses clarify the genetic distances among the five Egyptian clover cultivars. 
Keywords
Egyptian clover – RAPD – SRAP – molecular markers
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