Characterization of Antibacterial Peptides in Culex pipiens (Diptera: Culicidae) in Response to Bacillus sphaericus infection
Mohamed, D., Wassim, N., Habib, T., Khaled, H. (2023). Characterization of Antibacterial Peptides in Culex pipiens (Diptera: Culicidae) in Response to Bacillus sphaericus infection. EKB Journal Management System, 28(1), 33-42. doi: 10.21608/cat.2023.221522.1181
Doaa Mamdouh Mohamed; Nahla Wassim; Tito Habib; Howayda Khaled. "Characterization of Antibacterial Peptides in Culex pipiens (Diptera: Culicidae) in Response to Bacillus sphaericus infection". EKB Journal Management System, 28, 1, 2023, 33-42. doi: 10.21608/cat.2023.221522.1181
Mohamed, D., Wassim, N., Habib, T., Khaled, H. (2023). 'Characterization of Antibacterial Peptides in Culex pipiens (Diptera: Culicidae) in Response to Bacillus sphaericus infection', EKB Journal Management System, 28(1), pp. 33-42. doi: 10.21608/cat.2023.221522.1181
Mohamed, D., Wassim, N., Habib, T., Khaled, H. Characterization of Antibacterial Peptides in Culex pipiens (Diptera: Culicidae) in Response to Bacillus sphaericus infection. EKB Journal Management System, 2023; 28(1): 33-42. doi: 10.21608/cat.2023.221522.1181

Characterization of Antibacterial Peptides in Culex pipiens (Diptera: Culicidae) in Response to Bacillus sphaericus infection

Catrina: The International Journal of Environmental Sciences
Volume 28, Issue 1, July 2023, Page 33-42  XML PDF (676.74 K)
Document Type: Original Article
DOI: 10.21608/cat.2023.221522.1181
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Authors
Doaa Mamdouh Mohamed email 1; Nahla Wassim1; Tito Habib2; Howayda Khaled1
1Zoology Department, faculty of Science, Suez university, Suez, Egypt.
2Zoology Department, Faculty of Science, Sohag University, Sohag, Egypt.
Abstract
Abstract
Antimicrobial peptides (AMPs) play a crucial role in defending mosquitoes against micro-organisms. The objective of this study was to identify AMPs produced in the hemolymph of Culex (Cx.) pipiens mosquitoes following bacterial infection. Mosquito larvae were collected from the Nahia drainage conduit in Giza Governorate and treated with Bacillus sphaericus strain 2362. The lethal concentration (LC50 and LC80) values were determined at concentrations of 0.042 and 0.174 ppm, respectively. The protein profiles of both treated and untreated Cx. pipiens mosquito larvae were analyzed using SDS-PAGE in a Bio-Rad cell. The analysis revealed the presence of several polypeptides, including Megacin β, α, and γ forms (15, 29.2, and 39.8 kDa), Gambicin (7.5 kDa), Diptericin (9.4 kDa), Attacin (23.8 kDa), and Subtilisin (27.6 kDa). Additionally, B. sphaericus double toxin Bin A (41.9 kDa) and Bin B (51.6 kDa) were observed, which are synthesized during sporulation and co-crystallize within bacteria. Furthermore, soluble mosquitocidal toxins Mtx1 (98.6 kDa) and Mtx2 (31 kDa) produced during vegetative growth were identified. Lastly, the two-component crystalline toxins Cry48Aa1 (135 kDa) and Cry49Aa1 (52.8 kDa) were detected. Understanding the mechanism behind mosquito resistance to bacterial pathogens is essential for developing strategies to combat this resistance.
Keywords
Keywords: Antimicrobial peptides; Bacillus sphaericus2362; Culex pipiens
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