Morphological studies and immunohistochemical detection of leptin and s-100 protein on the uropygial gland of baladi duck | ||||
| Benha Veterinary Medical Journal | ||||
| Article 1, Volume 29, Issue 1, September 2015, Page 1-8 PDF (217.46 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2015.31778 | ||||
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| Author | ||||
| Rasha R. Beheiry | ||||
| Department of Histology and Cytology. Faculty of Veterinary Medicine, Zagazig University, zagazig, Egypt | ||||
| Abstract | ||||
| Immunoperoxidase methods for the demonstration of leptin and S-100 protein were applied in routinefixed paraffin sections on the uropygial gland of baladi duck. Intact uropygial glands from twenty adult healthy duck were used after slaughter. The glands were removed then the tissue specimens were fixed in 10% buffered neutral formalin. This study showed that the uropygial gland formed from two lobes, which were encapsulated by a connective tissue capsule. Each lobe composed of peripheral tubules and central tubules that separated by connective tissue septa. The epithelial cells of the secretory tubules were consisted of four layers, the germinative, intermediate, secretory and degenerative cells. Immunohistochemical examination revealed that the leptin immunoreactivity was distributed in both the peripheral and the central tubules. The leptin immunostaining was strong in the basement membrane, the cytoplasm of germinative cells, secretory cells and degenerative cells. In addition, strong reaction to leptin antibody was seen in the lumen or central cavity of central tubules. Additionally, S100 immunoreactivity was detected strong in the capsule and with moderate reaction on the cytoplasm of peripheral and central tubules. This study emphasized that the strong positive reaction to leptin immunostaining in the secretion of central tubules reflect the nature of secretion of the preen wax. In addition, the expression of S100 protein immunostaining may indicate specific functions. | ||||
| Keywords | ||||
| Duck; leptin; S100 protein; Uropygial gland | ||||
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