Biochemical Studies of Na+,K+-ATP ase and Acetylcholinesterase Sensitivity to Phenothrin and Thiodicarb Among Different Egyptian Field Populations of Spodoptera littoralis
Seham M, I. (2008). Biochemical Studies of Na+,K+-ATP ase and Acetylcholinesterase Sensitivity to Phenothrin and Thiodicarb Among Different Egyptian Field Populations of Spodoptera littoralis. EKB Journal Management System, 29(January-March), 26-35. doi: 10.21608/asejaiqjsae.2008.3179
Ismail Seham M. "Biochemical Studies of Na+,K+-ATP ase and Acetylcholinesterase Sensitivity to Phenothrin and Thiodicarb Among Different Egyptian Field Populations of Spodoptera littoralis". EKB Journal Management System, 29, January-March, 2008, 26-35. doi: 10.21608/asejaiqjsae.2008.3179
Seham M, I. (2008). 'Biochemical Studies of Na+,K+-ATP ase and Acetylcholinesterase Sensitivity to Phenothrin and Thiodicarb Among Different Egyptian Field Populations of Spodoptera littoralis', EKB Journal Management System, 29(January-March), pp. 26-35. doi: 10.21608/asejaiqjsae.2008.3179
Seham M, I. Biochemical Studies of Na+,K+-ATP ase and Acetylcholinesterase Sensitivity to Phenothrin and Thiodicarb Among Different Egyptian Field Populations of Spodoptera littoralis. EKB Journal Management System, 2008; 29(January-March): 26-35. doi: 10.21608/asejaiqjsae.2008.3179

Biochemical Studies of Na+,K+-ATP ase and Acetylcholinesterase Sensitivity to Phenothrin and Thiodicarb Among Different Egyptian Field Populations of Spodoptera littoralis

Alexandria Science Exchange Journal
Article 3, Volume 29, January-March - Serial Number 1, January 2008, Page 26-35  XML PDF (649.31 K)
Document Type: Original Article
DOI: 10.21608/asejaiqjsae.2008.3179
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Author
Ismail Seham M
Center Laboratory of Pesticides, Sabahia, Alexandria, A.R.C.
Abstract
Enzymatic activity of Na +,K+ - ATPase and AChE of cotton leafworm Spodoptera littoralis collected from different four Egyptian field populations ranged from heavily- sprayed fields and cultivated fields were investigated and compared with a laboratory susceptible population. The highest levels of Na +,K+ - ATPase and  AChE activities were found in Alexandria Governorate  Egypt. The moderate leveals was found in El -Boheira  Governorate, Egypt. Na + ,K+ - ATPase and AChE and were  isolated from brain of  S. littoralis  larvae (4th instar). The sensitivity of Na +,K+ - ATPase and AChE activity to Phenothrin and Thiodicarb respectively were measured by  the I50 values. The I50 values of Phenothrin on the Na +,K+ - ATPase activity were 0.01, 0.20, 0.36, 0.61 and 0.82μM for lab strain; Borg El- Arab; Abeis; Damanhour and Abou El- Matamir respectively. The I50 of Thiodicarb on AChE  activity were 0.22, 0.43, 0.54, 0.71 and 0.96μM for lab  strain and four field strains respectively. The inhibition  constant (Ki) values were determined for Na+,K+ - ATPase and AChE inhibitors. Values of Ki in the case of Phenothrin were 5, 18, 20, 30 and 45μM for lab strain; Borg El-Arab; Abeis; Damanhour and Abou El-Matamir respectively on Na+,K+ - ATPase activity. Similarly, Thiodicarb were 20, 28, 30, 40 and 50μM for lab strain and four field strains respectively on AChE activity. The results of the present study may add some forward steps to uses this enzymes indicate of effect this insecticides under study, in the IPM programs of the cotton leafworm.
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