Typing of Clostridium perfringens isolated from some meat products by using PCR
Abd Al- Tawab, A., El-Hofy, F., Khater, D., Kotb, M. (2015). Typing of Clostridium perfringens isolated from some meat products by using PCR. EKB Journal Management System, 29(1), 118-123. doi: 10.21608/bvmj.2015.31803
Ashraf A. Abd Al- Tawab; Fatma I. El-Hofy; Dalia F. Khater; Mohamed A. M. Kotb. "Typing of Clostridium perfringens isolated from some meat products by using PCR". EKB Journal Management System, 29, 1, 2015, 118-123. doi: 10.21608/bvmj.2015.31803
Abd Al- Tawab, A., El-Hofy, F., Khater, D., Kotb, M. (2015). 'Typing of Clostridium perfringens isolated from some meat products by using PCR', EKB Journal Management System, 29(1), pp. 118-123. doi: 10.21608/bvmj.2015.31803
Abd Al- Tawab, A., El-Hofy, F., Khater, D., Kotb, M. Typing of Clostridium perfringens isolated from some meat products by using PCR. EKB Journal Management System, 2015; 29(1): 118-123. doi: 10.21608/bvmj.2015.31803

Typing of Clostridium perfringens isolated from some meat products by using PCR

Benha Veterinary Medical Journal
Article 15, Volume 29, Issue 1, September 2015, Page 118-123  XML PDF (391.01 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2015.31803
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Authors
Ashraf A. Abd Al- Tawab1; Fatma I. El-Hofy1; Dalia F. Khater2; Mohamed A. M. Kotb2
1Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University
2Animal Health Research Institute, Tanta branch
Abstract
One hundred and twenty random samples of meat products from different supermarkets in El-Gharbia
governorate including minced meat, beef burger, sausage, luncheon (30 samples of each) were collected.
C. perfringenswas detected in meat products with an incidence 15%. The incidence of C.perfringens in
minced meat, sausage, beefburger and luncheon was 16.67%, 23.33%, 16.67% and 3.33%, respectively.
Typing of C.perfringens isolates revealed that the incidences of toxigenic and non-toxigenic strains were
83.33% and 16.67%, respectively. Typing of toxigenic strains of C.perfringens revealed that all isolates
were type (A). Polymerase chain reaction (PCR) was applied for detection of toxin genes and enterotoxin
of C.perfringens. All tested isolates were positive for alpha toxin gene with amplified PCR product of
402 bp and were cpe negative. Public health importance of C.perfringens isolates was discussed.
Keywords
Clostridium perfringens; PCR; meat products
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