Isolation, identification and pathotyping of Newcastle disease virus from chickens in Egypt
El-Bagoury, G., El-Habbaa, A., El-Adaway, S., El-Mahdy, S. (2015). Isolation, identification and pathotyping of Newcastle disease virus from chickens in Egypt. EKB Journal Management System, 29(1), 196-204. doi: 10.21608/bvmj.2015.31819
Gabr F. El-Bagoury; Ayman S. El-Habbaa; Samar F. El-Adaway; Susan S. El-Mahdy. "Isolation, identification and pathotyping of Newcastle disease virus from chickens in Egypt". EKB Journal Management System, 29, 1, 2015, 196-204. doi: 10.21608/bvmj.2015.31819
El-Bagoury, G., El-Habbaa, A., El-Adaway, S., El-Mahdy, S. (2015). 'Isolation, identification and pathotyping of Newcastle disease virus from chickens in Egypt', EKB Journal Management System, 29(1), pp. 196-204. doi: 10.21608/bvmj.2015.31819
El-Bagoury, G., El-Habbaa, A., El-Adaway, S., El-Mahdy, S. Isolation, identification and pathotyping of Newcastle disease virus from chickens in Egypt. EKB Journal Management System, 2015; 29(1): 196-204. doi: 10.21608/bvmj.2015.31819

Isolation, identification and pathotyping of Newcastle disease virus from chickens in Egypt

Benha Veterinary Medical Journal
Article 25, Volume 29, Issue 1, September 2015, Page 196-204  XML PDF (290.07 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2015.31819
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Authors
Gabr F. El-Bagoury1; Ayman S. El-Habbaa1; Samar F. El-Adaway2; Susan S. El-Mahdy2
1Department of Virology, Faculty of Veterinary Medicine, Benha University
2Central Laboratory for Evaluation of Veterinary Biologics, Abbassia, Cairo
Abstract
Egypt is endemic for Newcastle disease virus (NDV) with continuous long-lasting outbreaks causing
significant losses in the poultry industry. This study was designed to isolate and identify NDV from
different localities in Egypt (Giza, Gharbiya, Kalyobiya, Sharkia, Menofia, Fayoum and Minia) among
chicken flocks and estimate its virulence using Mean death time (MDT) and intra-cerebral pathogenicity
index (ICPI). Forty samples were collected from chickens either alive or dead showing clinical findings
and post-mortem lesions characteristic for NDV. Virus propagation in embryonated chicken eggs was
confirmed by hemagglutination (HA) test and identified by hemagglutination inhibition (HI) test using
NDV specific antiserum. The results indicated that 23 (57.5 %) out of 40 samples were NDV positive.
The isolate from Giza was velogenic with MDT of 48 hours and ICPI of 1.625. While the isolate from
Qualubiya was lentogenic with MDT of 96 hours and ICPI of 0.4375.These findings provide data on
biological pathotyping of NDV in chickens in Egypt and emphasize importance of NDV surveillance
for improving of strategies for the control of the disease
Keywords
NDV; MDT; ICPI; HI; virus isolation
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