The Potential Protective Impact of Selenium on Cyclophosphamide Induced Hepatotoxicity and Nephrotoxicity in Adult Male Albino Rats

Hussieny Mahmoud, Abeer Ramzy; Mostafa Arafa, Manar Hamed; Mohammed Farag, Dalia Abd Elmoain; Mohammed Ali, Asmaa Gamal; Megahed, Elham Elshawadfy

doi:10.21608/zumj.2023.236345.2891

	The Potential Protective Impact of Selenium on Cyclophosphamide Induced Hepatotoxicity and Nephrotoxicity in Adult Male Albino Rats
Zagazig University Medical Journal
Articles in Press, Accepted Manuscript, Available Online from 24 September 2023
Document Type: Original Article
DOI: 10.21608/zumj.2023.236345.2891
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Authors
Abeer Ramzy Hussieny Mahmoud¹; Manar Hamed Mostafa Arafa¹; Dalia Abd Elmoain Mohammed Farag²; Asmaa Gamal Mohammed Ali ³; Elham Elshawadfy Megahed¹
¹Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Zagazig University, Egypt
²Histology and Cell Biology Department, Faculty of Medicine, Zagazig University, Egypt
³Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Zagazig University, Egypt
Abstract
Background: Human exposure to cyclophosphamide (CP) is highly frequent because it is widely used chemotherapeutic and immunosuppressive. CP has potential toxic effects on liver and kidney that make it an issue of concern to public health. Selenium (Se) is an essential dietary element with a high nutritional value, immunomodulatory and antioxidant properties. This study aimed to evaluate the protective role of Se against toxic effects of CP in hepatic and renal tissues of adult male albino rats. Methods: The study was carried on 42 adult male albino rats were divided into 6 groups, 7 rats for each group. Group I (Negative control). group II (Positive control): divided into group IIA (Distilled water), group IIB (Normal saline), group III (Selenium), group IV (CP) and group V (CP + Se). The following markers were measured: serum ALT (Alanine aminotransferase), AST(Aspartate transaminase), LDH (Lactate dehydrogenase), Urea and Creatinine. Hepatic and renal tissue MDA (Malondialdehyde), PCC (Protein carbonyl content), catalase, GSH (Reduced glutathione) and IL-6 (Interleukin six). Histopathological and Immunohistochemical staining studies were demonstrated. Results: In CP administrated rats, serum ALT , AST, LDH , Urea and Creatinine levels increased. Also, CP induced elevation in hepatic and renal tissues MDA, PCC and IL-6 and a decrease in hepatic and renal tissues catalase and GSH. Histopathology and Immunohistochemical staining showed that: CP induced histological damages and strong immunoreaction decreased by co-treatment of Se. Conclusion: Administration of Se improved liver and kidney functions and histology beside improvement in oxidative stress and inflammation caused by CP.
Keywords
Selenium; cyclophosphamide; hepatotoxicity; nephrotoxicity; rats


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