Isolation and characterization of lytic bacteriophages specific for antibiotic-resistant Acinetobacter baumannii

Mahmoud, Essam Saad; Taha, Reda M.; Bassyouni, Rasha H; Ahmed, Fatma A.; Samhan, Farag A

doi:10.21608/ifjsis.2023.228796.1035

	Isolation and characterization of lytic bacteriophages specific for antibiotic-resistant Acinetobacter baumannii
Labyrinth: Fayoum Journal of Science and Interdisciplinary Studies
Volume 1, Issue 2, October 2023, Page 55-64 PDF (1021.19 K)
Document Type: Original full papers (regular papers)
DOI: 10.21608/ifjsis.2023.228796.1035
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Authors
Essam Saad Mahmoud ¹; Reda M. Taha²; Rasha H Bassyouni³; Fatma A. Ahmed³; Farag A Samhan⁴
¹Botany Department, Science Faculty, Fayoum University
²Botany Department, Faculty of Science, Fayoum University, Fayoum, Egypt
³Medical Microbiology and Immunology Department, Faculty of Medicine, Fayoum University, Egypt.
⁴Water and Wastewater Microbiology Department, National Research Centre, Dokki, Cairo, Egypt.
Abstract
Almost all conventional antibiotics are no longer effective against the common nosocomial infection Acinetobacter baumannii. Bacteriophage therapy is an effective alternative for bacterial infections resistant to many drugs. In the present study, a lytic bacteriophage, ФEAP, which infects A. baumannii and has a wide-ranging host range, was chosen for characterization. Phage ФEAP described in this research has the ability to lysis A. baumannii that resists 94 % of antibiotics (resist to 16 antibiotics from 17 have been used). A. baumannii isolated from a resistant wound abscess. When ФEAP alone incubated at different pH values and different temperatures, the phage was stable over a wide pH range (4 to 9) and at extreme temperatures (between 50°C and 60°C). In addition, ФEAP tolerates all concentrations of NaCl solutions used so it shows salinity tolerance and resistance to UV rays. ФEAP has a 94.4 nm icosahedral head and a 302 nm contractile tail. Pure concentrated phage was negatively stained and investigated using transmission electron microscopy (TEM). Electron micrographs showed that the phage belonged to a member of the Myoviridae family and the Caudovirales order.
Keywords
bacteriophage; Acinetobacter baumannii; Myoviridae; and Caudovirales


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