ASSESSMENT OF BIOCHEMICAL, HISTOLOGICAL CHANGES AND TISSUE EXPRESSION OF P53 AS INDICATOR OF EARLY POSTMORTEM INTERVAL IN ADULT MALE ALBINO RATS
kasem, S., Mohamed, S., Mohamed, S., Khalaf, M., Said, A. (2023). ASSESSMENT OF BIOCHEMICAL, HISTOLOGICAL CHANGES AND TISSUE EXPRESSION OF P53 AS INDICATOR OF EARLY POSTMORTEM INTERVAL IN ADULT MALE ALBINO RATS. EKB Journal Management System, 23(3), 97-111. doi: 10.21608/ejfsat.2023.211493.1290
Sara Elsayed kasem; Soheir Ali Mohamed; Samira Mahmoud Mohamed; Martena Osam Khalaf; Ahmed Mohamed Said. "ASSESSMENT OF BIOCHEMICAL, HISTOLOGICAL CHANGES AND TISSUE EXPRESSION OF P53 AS INDICATOR OF EARLY POSTMORTEM INTERVAL IN ADULT MALE ALBINO RATS". EKB Journal Management System, 23, 3, 2023, 97-111. doi: 10.21608/ejfsat.2023.211493.1290
kasem, S., Mohamed, S., Mohamed, S., Khalaf, M., Said, A. (2023). 'ASSESSMENT OF BIOCHEMICAL, HISTOLOGICAL CHANGES AND TISSUE EXPRESSION OF P53 AS INDICATOR OF EARLY POSTMORTEM INTERVAL IN ADULT MALE ALBINO RATS', EKB Journal Management System, 23(3), pp. 97-111. doi: 10.21608/ejfsat.2023.211493.1290
kasem, S., Mohamed, S., Mohamed, S., Khalaf, M., Said, A. ASSESSMENT OF BIOCHEMICAL, HISTOLOGICAL CHANGES AND TISSUE EXPRESSION OF P53 AS INDICATOR OF EARLY POSTMORTEM INTERVAL IN ADULT MALE ALBINO RATS. EKB Journal Management System, 2023; 23(3): 97-111. doi: 10.21608/ejfsat.2023.211493.1290

ASSESSMENT OF BIOCHEMICAL, HISTOLOGICAL CHANGES AND TISSUE EXPRESSION OF P53 AS INDICATOR OF EARLY POSTMORTEM INTERVAL IN ADULT MALE ALBINO RATS

The Egyptian Journal of Forensic Sciences and Applied Toxicology
Volume 23, Issue 3, September 2023, Page 97-111  XML PDF (1.37 MB)
Document Type: Original Article
DOI: 10.21608/ejfsat.2023.211493.1290
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Authors
Sara Elsayed kasem email 1; Soheir Ali Mohamed2; Samira Mahmoud Mohamed3; Martena Osam Khalaf4; Ahmed Mohamed Said5
1Forensic Medicine and Clinical Toxicology Department,Faculty of Medicine, Sohag University.Sohag, Egypt
2Department of Forensic Medicine and Clinical Toxicology , Faculty of Medicine, Sohag University, Egypt
3Department of Histology and Cell Biology, Faculty of Medicine, Sohag University, Egypt
4Department of Forensic Medicine and Clinical Toxicology , Faculty of Medicine, Sohag University, Egypt.
5forensic medicine clinical toxicology, faculty of medicine, sohag university, sohag, egypt
Abstract
Estimating the period since death is forensic medicine's primary priority. It assists other judicial inquiries in comprehending the situation and accounting for prospective offenders. Objectives: The current research is attempting to evaluate the role of various methods in the estimation of the postmortem interval by studying the biochemical changes in the blood (serum levels of uric acid, lactate, amylase, lipase, and insulin), histological changes, and anti-p53 antibody immunohistochemical expression in the tongue and pancreas. Methodology: Forty adult male Albino rats served as the subjects for the current investigation. According to varied postmortem intervals, the animals were randomly divided into four groups: 0 hours, 12 hours, 24 hours, and 48 hours after death. Results: The postmortem interval, serum uric acid, lactate, and lipase displayed a significant positive correlation. Time-dependent alterations could be observed in the pancreatic and tongue histological sections. Anti-p53 antibody immunohistochemistry expression increased progressively over the postmortem period. Conclusions: Determining how long has transpired after death is essential in forensic medicine. The serum lactate level is the most accurate of the studied biochemical parameters. Additionally, regression formulas using the optical density scores of the tongue and pancreas can be used to forecast PMI more accurately when combined.
Keywords
Postmortem interval; Postmortem biochemistry; Apoptosis; p53
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