Efficiency of Folic acid and Zinc chloride in ameliorating Arsenic-induced toxicity in salivary gland and taste buds of adult male albino rats | ||||
| Egyptian Dental Journal | ||||
| Volume 69, Issue 4 - Serial Number 3, October 2023, Page 2681-2696 PDF (5.1 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/edj.2023.218991.2614 | ||||
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| Authors | ||||
Alzahraa Ahmed alghriany   1; Alshaimaa Ahmed Alghriany 2; Eman Ahmed Negm 3; Maii Ibrahim Sholqamy 4
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| 1Lecturer of oral medicine,periodontology and diagnosis, Faculty of Dentistry, Assiut University, Assiut, Egypt | ||||
| 2Lecturer of Zoology and Entomology, Faculty of Science, Assiut University, Assiut, Egypt. | ||||
| 3Lecturer of Physiology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt | ||||
| 4Assistant professor of Oral and Maxillofacial Pathology – Faculty of Dentistry, Minia University, Egypt | ||||
| Abstract | ||||
| OBJECTIVE: Assessment of the effects of arsenic on the submandibular salivary gland and taste buds in rats, and how folic acid and zinc chloride ameliorate these effects. METHODOLOGY: Twenty-five rats were divided equally into 5 groups: (C); without any treatments, (HCl); got a daily oral dose of HCl for 15 days, (As); received 3 mg/kg b.w. orally of As III for 15 days. (As + Fa) and (As + Zn) received 0.1 mg/kg of folic acid and 2 mg/kg of Zncl2 respectively orally daily for 2 months after receiving As III. Plasma IL-6, IL-10, NO, and GSH measurements, Histological and immunohistochemical (GLUT-1 and cleaved caspase-3) examinations on submandibular salivary glands and taste buds were performed. RESULTS: Arsenic increased IL-6 and NO levels and decreased IL-10 and GSH levels significantly. Submandibular salivary gland and taste buds showed alterations as apoptosis and necrosis. Compared to control, salivary gland collagen fibers, GLUT-1, and taste buds cleaved caspase-3 increased significantly. Folic acid and zinc after arsenic exposure raised IL-10, GSH, and decreased IL-6 and NO significantly. Submandibular salivary glands and taste buds appeared nearly normal. Salivary gland collagen fibers, GLUT-1 immunoreaction, and taste buds cleaved caspase-3 immunoreaction decreased significantly versus arsenic group. CONCLUSION: Oxidative stress from arsenic damages taste buds and submandibular salivary gland via apoptosis, necrosis, and inflammation. Folic acid and zinc chloride minimize that damage. KEYWORDS: Arsenic, apoptosis, oxidative stress, folic acid, zinc chloride, healthcare. | ||||
| Keywords | ||||
| Arsenic; apoptosis; oxidative stress; folic acid; zinc chloride | ||||
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