The antibacterial and immunomodulatory effect of Zinc-oxide nanoparticles on pseudomonas aeruginosa infected fresh-water fish | ||||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||||
| Articles in Press, Accepted Manuscript, Available Online from 04 October 2023 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/scvmj.2023.237293.1143 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Nada Mehessen   1; Waleed Fathy Khalil 2; Abdel-Fattah Mohamed Mohamed Aly 3; Eman Ali Eldemerdash4
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| 1Assistant Lecturer, Pharmacology Dept., Faculty of Veterinary Medicine, Suez Canal University., Ismailia, Egypt | ||||
| 2Professor of Pharmacology Faculty of Veterinary Medicine, Suez Canal University | ||||
| 3Pharmacology Dept., Veterinary coolege, Suez Canal University., Ismailia, Egypt | ||||
| 4Free Veterinarian | ||||
| Abstract | ||||
| Nano-zinc oxide particles (ZnO NPs) are used in diverse industrial and commercial fields. In this study, 120 freshwater fish (Oreochromis niloticus) were divided into 4 groups, each in triplicate groups (n=10), and used to assess the pharmacological activity of ZnONPs as an anti-bacterial and immunomodulatory agent. Initially, the antibacterial activity was detected in vitro through the disk diffusion method. The results revealed a potent antibacterial activity, especially at 80 mg/kg of ZnONPs. Then, Pathogenicity test with pseudomonas aeruginosa was done. The clinical signs following the infection were also detected and the mortality rate was calculated. Administration of nano-zinc oxide (80mg/kg) showed potent ability to protect the fish from mortality induced by I/P injection of P. aeruginosa. Finally, blood samples were collected twice, on the 20th and 40th days, to detect the effect of ZnONPs on some immune parameters including granulocyte, monocytes, lymphocytes and white blood cells (WBCs) count. The results of the oral administration of both nano-zinc oxide and zinc acetate showed that there was a significant increase in lymphocyte, monocyte and WBC count from the 20th to 40th days in infected tilapia. In contrast, the results of granulocyte count revealed that the counts decreased from the 20th to 40th days in groups exposed to 80mg/kg ZnO NPs. In conclusion, ZnO-NPs showed significant antibacterial activity against P. aeruginosa-infected tilapia fish either in-vitro or in-vivo as well as increased the immunity of the fish against P. aeruginosa infection. | ||||
| Keywords | ||||
| Antibacterial; Immunity; Nile Tilapia; Oreochromis niloticus; Pseudomonas aeruginosa. | ||||
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