Susceptibility of Culex pipiens L. in Sharkia Governorate, Egypt to Chitin Synthesis Inhibitors and their Biochemical Characterizations | ||||
| Benha Journal of Applied Sciences | ||||
| Article 80, Volume 8, Issue 5, May 2023, Page 115-125 PDF (792.15 K) | ||||
| Document Type: Original Research Papers | ||||
| DOI: 10.21608/bjas.2023.320686 | ||||
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| Authors | ||||
| Mona F.A. El-Sitiny1; Mohammed E. Gad2; Hanem F. Khater* 3; Mohammed G. Mahmoud1 | ||||
| 1Plant Protection Department, Agricultural Faculty, Zagazig University, Egypt | ||||
| 2Zoology Department, Faculty of Science (Boys) Al-Azhar University, Naser City, Cairo11884, Egypt | ||||
| 3Parasitology Department, Faculty of Veterinary Medicine, Banha University, Egypt | ||||
| Abstract | ||||
| Culex pipiens is an important vector in Egypt. The tolerance levels of insect growth regulators (IGRs) on 4th instar larvae of Cx. pipiens collected from the Faqous region, Sharkia Governorate, Egypt, and their biochemical characterization were determined. The data indicated that LC50 values post-treatment (PT) of the field strain with diflubenzuron, novaluron, and lufenuron were 0.08, 0.60, and 0.16 μg/ml, respectively. Whereas the corresponding values for the laboratory strain were 0.07, 0.16, and 0.02 μg/ml, respectively. The relative tolerances of the field strain reached 1.14 (low), 3.75 (medium), and 8.00 (high) folds, respectively, when compared to the laboratory strain. The total protein levels in larvae treated with diflubenzuron, novaluron, and lufenuron and those of laboratory strain were 17.00, 20.00, 27.33, and 13.33 mg/g b.wt, respectively, whereas such values for the target enzyme of acetylcholine esterase, AChE, were 210.00, 283.33, 310.00, and 225.00 AChBr/min./g.b. wt, respectively. Their effect on the detoxifying enzymes reached 242.00, 386.67, 483.33, and 235.00 µ Meb min-1 mg-1 protein, respectively, for carboxylesterases; 28.00, 35.67, 62.33, and 22.00 µmol min-1 mg-1 protein in case of glutathione- S- transferase; 560.00, 723.33, 921.67, and 503.33 ηmol min-1 mg-1 protein, respectively, for α – esterase; and 128.33, 151.67, 175.00, and 131.67, ηmol min-1 mg-1 protein, respectively, for β – esterase. The tolerance levels for the applied IGRs revealed that the field strain from the Faqous region was highly tolerant to lufenuron, followed by novaluron, and diflubenzeron; therefore, diflubenzeron is the IGR of choice to be applied in such area. | ||||
| Keywords | ||||
| Lufenuron; diflubenzuron; Novaluron; Esterases; Acetylcholineesterase; Glutathione-S- transferase; Carboxylesterases; Total protein | ||||
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