IN VITRO CULTURE OF SOME GUAVA CULTIVARS | ||||
| Menoufia Journal of Plant Production | ||||
| Article 5, Volume 40, Issue 1, February 2015, Page 131-138 PDF (986.4 K) | ||||
| Document Type: original papers | ||||
| DOI: 10.21608/mjppf.2015.321145 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| M.R.M. Rabeh; S.A. Ahmed; A.A kasem; Basma S .Salama | ||||
| Hort.Dept., Fac. Agric., Minuofiya Univ., Shebin El-Kom. | ||||
| Abstract | ||||
| ABSTRACT: The experiment was carried out in the Plant Tissue Culture Lab.of the Tissue Culture Lab.of Hort.Dept., Faculty of Agric., Minoufiya Univ., Shebin El-Kom in cooperation with Tissue Culture Lab., of Plant Biotechnology Dept., Genetic Engineering and Biotechnology Research Institute, Sadat City University, during the years 2010 to 2013 to investigate the procedures of tissue culture technique for clonal propagation of Banaty, Sabahy and Mobaker (guava cultivars). After surface-sterilization, the plant materials (shoot tips) were cultured in Murashige and Skoog (MS-1962)mediumsupplemented with various plant growth substances that favour shooting.The best disinfestation procedure obtained when the plant materials were immersed in 0.5% sodium hypochlorite for 6-8 minutes, followed by dipping in 70% ethanol for 1- 4 minutes, then in 0.3% mercuric chloride for 1 minute. Varietal differences appeared by applying different treatments of the three guava cultivars. BA at 1.0 mg/l gave the best effect on shoot production per explant of the three guava cultivars. The better effects on shoot production were obtained when IBA at 0.02 to 2.0 mg/l was combined with BA at 1-2 mg/l. Varietal differences appeared by changing the concentrations of the growth substances used. | ||||
| Keywords | ||||
| Surface-sterilization; Proliferation; Guava; Tissue culture | ||||
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