Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions
Saad, G., Hassanein, A., El-Deep, B., Salem, J., Farghal, Z. (2023). Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions. EKB Journal Management System, 31(1), 16-27. doi: 10.21608/jesj.2023.237831.1059
Ghada Kamel Saad; Ahmed M. Hassanein; Bahig A El-Deep; Jehan M Salem; Zeinab E Farghal. "Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions". EKB Journal Management System, 31, 1, 2023, 16-27. doi: 10.21608/jesj.2023.237831.1059
Saad, G., Hassanein, A., El-Deep, B., Salem, J., Farghal, Z. (2023). 'Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions', EKB Journal Management System, 31(1), pp. 16-27. doi: 10.21608/jesj.2023.237831.1059
Saad, G., Hassanein, A., El-Deep, B., Salem, J., Farghal, Z. Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions. EKB Journal Management System, 2023; 31(1): 16-27. doi: 10.21608/jesj.2023.237831.1059

Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions

Journal of Environmental Studies
Volume 31, Issue 1, September 2023, Page 16-27  XML PDF (698.38 K)
Document Type: High quality original papers
DOI: 10.21608/jesj.2023.237831.1059
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Authors
Ghada Kamel Saad email 1; Ahmed M. Hassanein2; Bahig A El-Deep1; Jehan M Salem3; Zeinab E Farghal4
1Botany and Microbiology Department, Faculty of Science, Sohag University
2Central Laboratory of Genetic Engineering, Botany and Microbiology Department, Faculty of Science, Sohag University, P.O. 82524, Sohag, Egypt.
3Botany and Microbiology Department, Faculty of Science, Sohag University, Sohag, Egypt
4Botany and Microbiology Department, Faculty of Science, Sohag University, Sohag, Egypt,
Abstract
Transfer banana plantlet from in vitro to ex vitro conditions is essential prerequisite in micropropagation. In vitro grown plantlets are unable to cope with soil environment. To avoid these challenges, the plant must be acclimatized according to a protocol that takes into account the economics of the plant concerned. Banana shoot culture was established on MS medium supplemented with 5 mg/l BAP and 30 g/l sucrose. Shoot cuttings were subjected for in vitro root formation on half strength MS medium containing 1 mg/l IBA. Plantlets were acclimatized under tissue culture room conditions where they were able to repair their physiological disorders and cope ex vitro conditions. Disorder repair processes were better if plantlets were treated with chitosan. When chitosan was used, efficiency of acclimatization increased during all acclimatization periods due to the increase in plant growth parameters, water content and ROS scavenging through the increase in activities of antioxidant enzymes such as superoxide dismutase and peroxidase. To save time and costs, root formation and acclimatization were combined in one step under transparent plastic bags (relative humidity 87%) in tissue culture room (25 oC) using ex vitro- hydroponic or soil-root formation procedure. Survival frequency was 100% when root formation was induced using 1 mg/l IBA and relative humidity was gradually decreased. Plantlets were transplanted in soil without application of in vitro or ex vitro root formation procedures died in five days.
Keywords
Acclimatization; antioxidant; Musa; chitosan; micropropagation
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