Assessment of Vascular Endothelial Growth Factor mRNA and miR-200b Levels in Type 2 Diabetes Mellites in Correlation with Clinicopathological and Anatomical Distribution of Diabetic Foot Ulcer | ||||
Zagazig University Medical Journal | ||||
Article 18, Volume 30, Issue 3, May and June 2024, Page 767-776 PDF (790.47 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/zumj.2023.240999.2937 | ||||
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Authors | ||||
Nearmeen Rashad ![]() ![]() | ||||
1zagazig university | ||||
2Internal Medicine, Zagazig University | ||||
3Assistant professor of Internal Medicine and Nephrology Faculty of Medicine - Zagazig University | ||||
4Lecturer of Internal Medicine, Faculty of Medicine, Zagazig University | ||||
5faculty of medicine, zagazig university | ||||
6Tropical medicine ,Zagazig university | ||||
7Medical Microbiology and Immunology | ||||
8Department of Human Anatomy and Embryology, Faculty of Medicine, Zagazig University, Egypt | ||||
9Clinical Pathology department,Research Institute of ophthalmology | ||||
10Internal Medicine Department, Faculty of Medicine, Zagazig University, Egypt | ||||
Abstract | ||||
Background: Diabetic foot ulcer (DFU) is one of the most distressing complications in patients with type 2 diabetes mellitus (T2DM). Vascular endothelial growth factor (VEGF) is a strong angiogenic factor associated with wound healing and development. Aim: This study aimed to investigate the miR-200b and VEGF mRNA levels in patients with T2DM and to discover their relations with the clinicopathological and anatomical distribution of DFU. Methods: This case-control study enrolled 70 patients with T2DM and 70 healthy subjects as controls. Physical and neurological examination to assess the Wagner classification. RT-PCR was done to assess miR-200b and VEGF mRNA expression levels. Results: there were significantly higher values of VEGF mRNA levels in patients with DFU (3.13±1.87) compared to patients without DFU (2.41±0.197) and controls (1.07±0.363), with P value ˂0.001* Additionally, miR-200b levels were significantly higher in patients with DFU (3.48±1.47) compared to patients without DFU (2.94±0.187) and controls (1.13±0.37), with P value ˂0.001*. The power of VEGF mRNA and miR-200b levels as a diagnostic marker for DFU were projected (2.5 and 3.1, respectively), which yielded a sensitivity of (77.1% and 74.3, respectively) and a specificity of (77.3% and 75.2%), with the AUC at (0.886 and 0.845, respectively) . The size of DFU in cm was 3.5±0.421 and the number of ulcers was 2.45±0.433. Conclusion: the VEGF mRNA and miR-200b levels were significantly higher in patients with T2DM, particularly those with DFU. Consequently, we believed that VEGF mRNA and miR-200b may serve as promising predictive biomarkers for diabetes and DFU. | ||||
Keywords | ||||
VEGF mRNA; miR-200b; Diabetic foot ulcer; anatomical distribution; ABI | ||||
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