RELATION BETWEEN DENTIN SCAFFOLD/EDTA EXPOSURE TIME DURATION AND THE ENDOGENOUS BONE MORPHOGENIC PROTEIN 7 RELEASE
mohammed, I., Elbackly, R., Badr, A. (2023). RELATION BETWEEN DENTIN SCAFFOLD/EDTA EXPOSURE TIME DURATION AND THE ENDOGENOUS BONE MORPHOGENIC PROTEIN 7 RELEASE. EKB Journal Management System, 69(4), 3261-3267. doi: 10.21608/edj.2023.225162.2655
Ibrahim Abdalmonem mohammed; Rania Mohmoud Elbackly; Amany El-Said Badr. "RELATION BETWEEN DENTIN SCAFFOLD/EDTA EXPOSURE TIME DURATION AND THE ENDOGENOUS BONE MORPHOGENIC PROTEIN 7 RELEASE". EKB Journal Management System, 69, 4, 2023, 3261-3267. doi: 10.21608/edj.2023.225162.2655
mohammed, I., Elbackly, R., Badr, A. (2023). 'RELATION BETWEEN DENTIN SCAFFOLD/EDTA EXPOSURE TIME DURATION AND THE ENDOGENOUS BONE MORPHOGENIC PROTEIN 7 RELEASE', EKB Journal Management System, 69(4), pp. 3261-3267. doi: 10.21608/edj.2023.225162.2655
mohammed, I., Elbackly, R., Badr, A. RELATION BETWEEN DENTIN SCAFFOLD/EDTA EXPOSURE TIME DURATION AND THE ENDOGENOUS BONE MORPHOGENIC PROTEIN 7 RELEASE. EKB Journal Management System, 2023; 69(4): 3261-3267. doi: 10.21608/edj.2023.225162.2655

RELATION BETWEEN DENTIN SCAFFOLD/EDTA EXPOSURE TIME DURATION AND THE ENDOGENOUS BONE MORPHOGENIC PROTEIN 7 RELEASE

Egyptian Dental Journal
Volume 69, Issue 4 - Serial Number 5, October 2023, Page 3261-3267  XML PDF (1.33 MB)
Document Type: Original Article
DOI: 10.21608/edj.2023.225162.2655
View on SCiNiTO View on SCiNiTO
Authors
Ibrahim Abdalmonem mohammed email orcid 1; Rania Mohmoud Elbacklyorcid 2; Amany El-Said Badrorcid 3
1Msc Student, Endodontics Department, Faculty of Dentistry, Mansoura University, Mansoura, Egypt
2Professor, Endodontic Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt
3Professor, Head of Department of Endodontic, Faculty of Dentistry, Mansoura University, Mansoura, Egypt
Abstract
Abstract
Aim: to demonstrate the amount of endogenous bone morphogenic protein 7 release under the effect of different times of EDTA exposure.
Material and methods: Dentin scaffolds were randomly assigned to the following groups (n = 4). Group 1 received 17 % EDTA for 5 minutes; Group 2 received 17 % EDTA for 10 minutes; Group 3 received 17 % EDTA for 15 minutes and distilled water was used as a control in Group 4. After each treatment, ELISA was used to quantify the amount of BMP7 liberated from the dentin scaffolds after pre-treatment with EDTA for 5, 10, and 15 minutes. The scaffolds were analyzed under SEM to investigate DPSCs attachment, morphology, the opening of dentinal tubules, and smear layer removal in the different groups.
Results: Dentin scaffolds conditioned with 17% EDTA for 10 and 15 min showed the maximum degrees of enlargement in dentinal tubules and smear layer removal, and revealed higher DPSCs attachment than the control group (p < 0.001). Moreover, dentin treated with 17% EDTA for 10 min manifested higher concentrations of BMP7 release than the unconditioned dentin (p < 0.05). Using scanning electron microscopy, scaffold treated with EDTA for 10 min presented the highest degrees of enlargement in dentinal tubules and smear layer removal than dentin treated with EDTA for 5 or 15 min.
Conclusion: maximum endogenous bone morphogenic protein 7 release was obtained when dentin scaffolds were treated with 17% EDTA for a period of 10 minutes.
Key word: DPSCs, dentin scaffold, EDTA, BMP-7.
Keywords
dental pulp stem cells; dentin scaffold; EDTA; BMP-7
Statistics
Article View: 55
PDF Download: 64