The protective role of selenium against acrylamide-induced cerebellar neurotoxicity in adult male albino rats | ||||
| Egyptian Journal of Histology | ||||
| Articles in Press, Accepted Manuscript, Available Online from 26 October 2023 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2023.226438.1930 | ||||
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| Authors | ||||
Alaa M. Abdullah  1; mohamed El-Badry mohamed2; Manal H. AL Badawi 3; Alsayed A. Abdelhady 4
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| 1Anatomy departmant, faculty of medicine, Helwan University, Helwan, Egypt | ||||
| 2Human Anatomy and Embryology Department, Faculty of Medicine, Assiut University | ||||
| 3faculty of medicine Helan univercity | ||||
| 4Anatomy and Embryology Faculty of Medicine Helwan University | ||||
| Abstract | ||||
| Background: Cerebellum is a part of the brain which has about a half of the total number of neurons within the CNS. Acrylamide is a chemical compound that is produced naturally in food as a result of high-temperature cooking. It is a potent neurotoxin. Selenium is an essential element to the good brain function. Aim of the Work: To evaluate the effect of Acrylamide and the possible protective role of Selenium on the cerebellum of male albino rats. Material and Methods: A total number of 40 male albino rats weighing 250 gm, aging 6 months were used in the study. They were divided into 4 main groups (10 rats each). Group I, the control group, Group II received ACR intraperitoneal. Group III received ACR intraperitoneal and Se orally. Group IV received Se orally. The regimen continued for 4 weeks. The rats were anaesthetized and the cerebellum was extracted, dissected and processed for the light, the TEM examination and morphometric measurements. Results: The light microscopic study of the group II revealed distorted Purkinje cells with pyknotic nuclei and irregularly separated white matter nerve fibers, but in group III, Purkinje cells were similar to the control group with disperse shrunken Purkinje cells. The TEM examination of the group II revealed irregular shrunken Purkinje cells with dark nuclei with distorted cytoplasmic organelles and shrunken granule cells comparing to group III which had normal cytoplasmic organelles of Purkinje and granule cells with few cells with euchromatic nuclei and irregular outline. The immunoreaction for GFAP was strong in group II in comparing to moderate in group III and weak in group IV. Conclusion: Co-administration of selenium with acrylamide minimize to a great extent the harmful effects of acrylamide on the cerebellum of rats. | ||||
| Keywords | ||||
| Albino rats; cerebellum; acrylamide; selenium | ||||
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