Ameliorating Effects of Kaempferol on Buffalo Oocytes Developmental Competence (Part- I)
Bahgat, H., Abdel-Ghafar, A., Badr, M., shafik, B., Azab, R., Ramadan, S., Mohamed, M. (2023). Ameliorating Effects of Kaempferol on Buffalo Oocytes Developmental Competence (Part- I). EKB Journal Management System, 45(1), 60-63. doi: 10.21608/bvmj.2023.221684.1677
Hagar S. Bahgat; Alaa Abdel-Ghafar; Magdy Ramadan Badr; Basant shafik; Rasha El-sayed Azab; Sherif Ramadan; Mohamed El-Raey Mohamed. "Ameliorating Effects of Kaempferol on Buffalo Oocytes Developmental Competence (Part- I)". EKB Journal Management System, 45, 1, 2023, 60-63. doi: 10.21608/bvmj.2023.221684.1677
Bahgat, H., Abdel-Ghafar, A., Badr, M., shafik, B., Azab, R., Ramadan, S., Mohamed, M. (2023). 'Ameliorating Effects of Kaempferol on Buffalo Oocytes Developmental Competence (Part- I)', EKB Journal Management System, 45(1), pp. 60-63. doi: 10.21608/bvmj.2023.221684.1677
Bahgat, H., Abdel-Ghafar, A., Badr, M., shafik, B., Azab, R., Ramadan, S., Mohamed, M. Ameliorating Effects of Kaempferol on Buffalo Oocytes Developmental Competence (Part- I). EKB Journal Management System, 2023; 45(1): 60-63. doi: 10.21608/bvmj.2023.221684.1677

Ameliorating Effects of Kaempferol on Buffalo Oocytes Developmental Competence (Part- I)

Benha Veterinary Medical Journal
Volume 45, Issue 1, October 2023, Page 60-63  XML PDF (869.22 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2023.221684.1677
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Authors
Hagar S. Bahgat1; Alaa Abdel-Ghafar2; Magdy Ramadan Badr3; Basant shafik4; Rasha El-sayed Azab5; Sherif Ramadan4; Mohamed El-Raey Mohamed email orcid 1
1Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, P.O. 13736, Tokh, Kaliobia, Egypt.
2Theriogenology department -benha university- faculty of veterinary medicine.
3Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute, Agriculture Research Center, Al Haram, P. O. 12556, Giza, Egypt.
4Animal Wealth Development Department, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt.
5Department of Physiology, Faculty of Veterinary Medicine, Benha University, P.O. 13736, Tokh, Kaliobia, Egypt.
Abstract
To our knowledge, the cytoprotective roles of Kaempferol on the in vitro matured buffalo oocytes and embryos are still unknown. The current research was conducted to investigate the ideal dose of kaempferol that could be used safely in the vitro embryo production technology of buffalo as well as investigates the kaempferol effects on buffalos, cumulus cell expansion, in vitro maturation, penetration, fertilization, cleavage, morula, and blastocyst rates. Tissue culture media-199 supplemented with Kaempferol at 0, 5, 10, and 15 µg/ml was used to mature cumulus-oocyte complexes (COCs). The current study revealed that kaempferol supplementation at 10 µg/ml in the in vitro maturation medium of buffalo oocytes significantly improved cumulus cell expansion (75.33±1.75%), in vitro maturation (87.08±1.16%), penetration (78.40±3.75%), fertilization (42.80±1.15%), cleavage (28.20±3.51%), morula (21.70±2.65%), and blastocyst rates (19.90±2.45%) of buffalos. In conclusion, the presence of kaempferol in buffalo oocytes maturation medium is potentially important to regulate its developmental competence. Moreover, 10µg/ml of Kaempferol is an ideal promising dose that could be used safely to augment the in vitro embryo production technology of buffalo.
Keywords
Kaempferol; Buffalo; Oocyte; Developmental; Competence
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