Modulatory effects of experimentally induced hyperthyroidism and melatonin on thymus gland immune function in male rat | ||||
| Benha Veterinary Medical Journal | ||||
| Volume 45, Issue 1, October 2023, Page 84-88 PDF (1.35 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2023.216622.1670 | ||||
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| Authors | ||||
Sara Habk  1; Mohamed Azab1; Randa Ismail1; Abeer Nafeaa1; Saad Shousha 2
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| 1Department of Physiology, Faculty of Veterinary Medicine, Benha University, Egypt | ||||
| 2a Department of Physiology, Faculty of Veterinary Medicine, Benha University, Egypt b Department of Biomedical Sciences, College of Veterinary Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia | ||||
| Abstract | ||||
| The current study aimed to investigate how L-thyroxine-induced hyperthyroidism and melatonin affect the immune system. The rats were divided into four groups, each with ten rats. The rats received the following treatments for four weeks: group I, control group; group II, melatonin group, treated with melatonin; group III, hyperthyroid group, treated with L-thyroxine; and group IV, hyperthyroid + melatonin group, treated with L-thyroxine and melatonin. Serum total T3 and T4 levels, CD4+ and CD8+ T lymphocytes analysis using flow cytometry, and relative gene expression of TNF-α, IL-10, IL-2, and IFN-γ were evaluated. The results revealed that hyperthyroidism substantially increased CD4+ T cells while not affecting CD8+ T cells percentage. Moreover, TNF-α, IL-2, and IFN-γ expressions were significantly upregulated in hyperthyroid rats, whereas IL-10 was not significantly altered. Melatonin administration to hyperthyroid rats significantly decreased thyroid hormone levels, increased CD4+ and CD8+ T cells percentage, upregulated IL-10, IL-2, and IFN-γ gene expression, and downregulated TNF-α gene expression. When compared to controls, the melatonin group exhibited a substantially higher percentage of CD4+ and CD8+ T cells and elevated levels of IL-10, IL-2, and IFN-γ gene expression; however, TNF-α gene expression had not changed appreciably. Therefore, it might be concluded that L-thyroxine and melatonin induce T lymphocyte proliferation and that melatonin also has anti-inflammatory properties. | ||||
| Keywords | ||||
| Hyperthyroidism; L-thyroxine; Melatonin; T lymphocytes; Immune system | ||||
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