The potential contribution of food contact surfaces to the incidence of Escherichia coli in Egyptian meat outlets, as well as their biofilm and pathogenicity characteristics.
Saad, R., Sabike, I., Abo EL-Roos, N., Edris, S., Elsabagh, R. (2023). The potential contribution of food contact surfaces to the incidence of Escherichia coli in Egyptian meat outlets, as well as their biofilm and pathogenicity characteristics.. EKB Journal Management System, 45(1), 167-171. doi: 10.21608/bvmj.2023.225650.1694
Raghda Ebrahim Saad; Islam Ibrahim Sabike; Nahla S. Abo EL-Roos; shimaa Edris; Rasha Elsabagh. "The potential contribution of food contact surfaces to the incidence of Escherichia coli in Egyptian meat outlets, as well as their biofilm and pathogenicity characteristics.". EKB Journal Management System, 45, 1, 2023, 167-171. doi: 10.21608/bvmj.2023.225650.1694
Saad, R., Sabike, I., Abo EL-Roos, N., Edris, S., Elsabagh, R. (2023). 'The potential contribution of food contact surfaces to the incidence of Escherichia coli in Egyptian meat outlets, as well as their biofilm and pathogenicity characteristics.', EKB Journal Management System, 45(1), pp. 167-171. doi: 10.21608/bvmj.2023.225650.1694
Saad, R., Sabike, I., Abo EL-Roos, N., Edris, S., Elsabagh, R. The potential contribution of food contact surfaces to the incidence of Escherichia coli in Egyptian meat outlets, as well as their biofilm and pathogenicity characteristics.. EKB Journal Management System, 2023; 45(1): 167-171. doi: 10.21608/bvmj.2023.225650.1694

The potential contribution of food contact surfaces to the incidence of Escherichia coli in Egyptian meat outlets, as well as their biofilm and pathogenicity characteristics.

Benha Veterinary Medical Journal
Volume 45, Issue 1, October 2023, Page 167-171  XML PDF (8.01 MB)
Document Type: Original Article
DOI: 10.21608/bvmj.2023.225650.1694
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Authors
Raghda Ebrahim Saad1; Islam Ibrahim Sabike email orcid 2; Nahla S. Abo EL-Roos3; shimaa Edrisorcid 2; Rasha Elsabaghorcid 2
11 Food Hygiene and Control, Dept., Fac. Vet. Med., Benha University, Egypt. 2Animal health research institute, Shibin El Kom, Menofia, Egypt
2Food Hygiene and Control, Dept., Fac. Vet. Med., Benha University, Egypt.
3Animal Health research institute, Shebin El koom branch
Abstract
The study aimed to determine Escherichiacoliprevalence and their virulence and biofilm formation traits, in meat-contact and equipment surfaces at butcher shops, and supermarkets in Al-Menofia governorate, Egypt. One hundredswabs were collected from ten butcher shops and ten supermarkets (50 ofeach). The isolation and identification of targeted pathogens were conducted using standard culturing methods, biochemically using the VITEK2 compact system, and PCR techniques. PCR was used to investigate the genetic components that promote virulence and biofilm formation traits.Escherichiacoli was confirmed in 20% of swabbed samples. Targeted pathogen detection rates were higher from butchers than from supermarkets (24%vs. 16%) (P > 0.05). Eight serotypes and four pathotypes of Escherichiacoli were identified with enterohaemorrhagic accounting for 55%. Six of the eight Escherichiacoli isolates co-expressed Crl in all CsgA biofilm genes, with four, EHEC, also expressing Stx1.Strong virulence and a high prevalence of biofilm-producing components in isolated bacteria, together with popular Egyptian purchasing behaviors, raise concerns about the potential contamination of meat products and, eventually, humans.
Keywords
Escherichia coli; virulence and biofilm traits; meat contact/equipment surfaces
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