Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) technique for identification of Pseudomonas aeruginosa isolated from broilers and human.
Elsayed, M., Awad, A., El-hofy, F., Rizk, A. (2023). Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) technique for identification of Pseudomonas aeruginosa isolated from broilers and human.. EKB Journal Management System, 45(1), 204-208. doi: 10.21608/bvmj.2023.224084.1685
Marwa Mohamed Elsayed; Ashraf Awad; Fatma I. El-hofy; Amira Mohamed Rizk. "Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) technique for identification of Pseudomonas aeruginosa isolated from broilers and human.". EKB Journal Management System, 45, 1, 2023, 204-208. doi: 10.21608/bvmj.2023.224084.1685
Elsayed, M., Awad, A., El-hofy, F., Rizk, A. (2023). 'Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) technique for identification of Pseudomonas aeruginosa isolated from broilers and human.', EKB Journal Management System, 45(1), pp. 204-208. doi: 10.21608/bvmj.2023.224084.1685
Elsayed, M., Awad, A., El-hofy, F., Rizk, A. Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) technique for identification of Pseudomonas aeruginosa isolated from broilers and human.. EKB Journal Management System, 2023; 45(1): 204-208. doi: 10.21608/bvmj.2023.224084.1685

Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) technique for identification of Pseudomonas aeruginosa isolated from broilers and human.

Benha Veterinary Medical Journal
Volume 45, Issue 1, October 2023, Page 204-208  XML PDF (952.7 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2023.224084.1685
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Authors
Marwa Mohamed Elsayed email orcid 1; Ashraf Awadorcid 2; Fatma I. El-hofy3; Amira Mohamed Rizk3
1Bacteriology, Immunology and Mycology , Faculty of veterinary medicine , Benha university , Egypt
2Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University
3Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University, Qalyubia 13518, Egypt.
Abstract
Pseudomonas aeruginosa is an opportunistic pathogen that is ubiquitously distributed throughout the environment, particularly in moist habitats. It causes sever economic losses in poultry industry. It also causes serious diseases in human especially in nosocomial patients lead to high death rates. The main objective in this study is to explain the prevalence rates of P. aeruginosa in broilers and human. So, the samples were collected from 100 broiler chicken at different age groups; one day old chicks n=50, 14 days old chickens n=25 and 28 days old chickens n=25. From each chick, different organs were collected (liver, gall bladder, intestine, lungs, cloacal swabs, n=100 each). Yolk sac samples were collected only from one day old chicks (n=50).Thirty samples were collected from human: urine samples from patients complain from urinary tract infections (UTIs) (n=17) and pus swabs from abscesses (n=13) at Qalyubia Governorate , Egypt. The results of bacteriological examinations revealed that P. aeruginosa was recovered from; 47/100 chickens (47%), 5/17 (29.41%) urine samples, and 3/13 (23.07%) pus swabs. A Total of 55 isolates (47 isolates from broilers and 8 isolates from human) were identified by MALDI-TOF MS. It concluded that MALDI-TOF MS identification were 24/55 (43.63%), while by conventional method were 55/100 (55%) ; ( from broilers and human) , so MALDI-TOF MS is more accurate than conventional method.
Keywords
Keywords : Pseudomonas aeruginosa; Broilers; (UTIs) and MALDI-TOF MS
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