Citharexylum spinosum promotes antioxidant, anti-inflammatory, and anti-acetylcholinesterase activities
Saad, S., Abd-Alla, H., Aly, H., Shalaby, N., Afify, A., Ali, H., Abdel-Moein, N. (2024). Citharexylum spinosum promotes antioxidant, anti-inflammatory, and anti-acetylcholinesterase activities. EKB Journal Management System, 67(5), 63-75. doi: 10.21608/ejchem.2023.235755.8594
Safaa A. Saad; Howaida I. Abd-Alla; Hanan F. Aly; Nagwa M. M. Shalaby; Abd El-Moneim M. R. Afify; Hanaa F. M. Ali; Nadia M. Abdel-Moein. "Citharexylum spinosum promotes antioxidant, anti-inflammatory, and anti-acetylcholinesterase activities". EKB Journal Management System, 67, 5, 2024, 63-75. doi: 10.21608/ejchem.2023.235755.8594
Saad, S., Abd-Alla, H., Aly, H., Shalaby, N., Afify, A., Ali, H., Abdel-Moein, N. (2024). 'Citharexylum spinosum promotes antioxidant, anti-inflammatory, and anti-acetylcholinesterase activities', EKB Journal Management System, 67(5), pp. 63-75. doi: 10.21608/ejchem.2023.235755.8594
Saad, S., Abd-Alla, H., Aly, H., Shalaby, N., Afify, A., Ali, H., Abdel-Moein, N. Citharexylum spinosum promotes antioxidant, anti-inflammatory, and anti-acetylcholinesterase activities. EKB Journal Management System, 2024; 67(5): 63-75. doi: 10.21608/ejchem.2023.235755.8594

Citharexylum spinosum promotes antioxidant, anti-inflammatory, and anti-acetylcholinesterase activities

Egyptian Journal of Chemistry
Volume 67, Issue 5, May 2024, Page 63-75  XML PDF (694.86 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2023.235755.8594
View on SCiNiTO View on SCiNiTO
Authors
Safaa A. Saad1; Howaida I. Abd-Alla email orcid 1; Hanan F. Aly email orcid 2; Nagwa M. M. Shalabyorcid 1; Abd El-Moneim M. R. Afify3; Hanaa F. M. Ali3; Nadia M. Abdel-Moein3
1Natural Compounds Chemistry Department, National Research Centre, Dokki 12622, Giza, Egypt
2Therapeutic Chemistry Department, National Research Centre, Dokki 12622, Giza, Egypt
3Biochemistry Department, Faculty of Agriculture, Cairo University, Egypt
Abstract
Citharexylum spinosum leaves extract; n-hexane (n-Hex), n-hexane:benzene (n-Hex:Bz), chloroform (CHCl3), ethyl acetate (EtoAc), n-butanol (n-BuOH) and ethanol:H2O50% (Eth:H2O 50%) were evaluated for their anti-acetylcholinesterase, antioxidant and anti-inflammatory activities. The highest anti-acetylcholinesterase activity was obtained by n-BuOH extract followed by n-Hex:Bz and Eth:H2O 50% (IC50; 0.60, 0.80 and 1.86 µg/ml, respectively). The antioxidant results showed that, n-BuOH exhibited the highest DPPH● scavenging activity (85.91, 87.32, 56.33 and 39.43%) at different concentrations (1-100µg/µl), and 391.71±3.2μg/ml for H2O2 antioxidant method. While Eth:H2O 50% extract exhibited the highest antioxidant by reducing power method, FRAP and ABTS● scavenging activity (87.94%, 1382.29µmol of Fe2+/g sample and 20.01mmol Trolox/g sample, respectively). The highest anti-inflammatory effect obtained by n-BuOH extract followed by Eth:H2O 50% (IC50 74.50 and 109.80μg/ml, respectively). The screening of bimolecular components was carried out for n-BuOH and Eth:H2O 50% extracts by using LC-MS/MS. The LC-MS/MS profile showed the presence of organic acids; chlorogenic, gallic, caffeic, coumaric, ellagic, cinnamic, 3.4-dihydroxybenzoic, methyl gallate, syringic and ferulic) and flavonoids (rutin, vanillin, naringenin, querectin and luteolin). The current study aimed to for the first time to evaluate the anti-cholinesterase activity of C. spinosum leaves besides, the determination of the antioxidant and anti-inflammatory activities by different methods.
Keywords
Citharexylum spinosum; successive extracts; cholinesterase inhibitory activity; antioxidant; anti-inflammatory; LC-MS/MS profile
Statistics
Article View: 95
PDF Download: 48