New Chitinolytic Alcaligenes Species Strains Isolated from Shrimp Shells | ||||
| Egyptian Journal of Aquatic Biology and Fisheries | ||||
| Article 67, Volume 27, Issue 5, September and October 2023, Page 1191-1205 PDF (574.13 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejabf.2023.323781 | ||||
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| Author | ||||
| Mai Ibrahim et al. | ||||
| Abstract | ||||
| Chitinases have received attention in the last decades because of their wide applications in medicine, biotechnology, agriculture, waste management, and industrial applications such as food quality enhancers and biopesticides. The objectives of this research were to isolate and identify potentially chitinolytic bacterial species from shrimp shells and analyze their potency for chitinase production. The research consisted of a screening of chitinolytic bacteria based on the zone of clearance on a colored agar plate and enzyme activity assay using the dinitrosalicylic acid (DNS) method. Five bacterial isolates of chitinolytic bacteria showed high chitinolytic activity. Molecular identification of bacterial isolates was based on polymerase chain reaction (PCR) of 16S rRNA universal primers and sequences analysis. The isolates identified as chi1 and chi2 were Alcaligenes faecalis, while chi3, chi4, and chi5 were Alcaligenes ammonioxydans, Alcaligenes aquatilis and E. coli, respectively, with 99% similarity. Two of them are the high producers of chitinase, chi4 Alcaligenes aquatilis (4.5 U/ml) andchi3 Alcaligenes ammonioxydans (4.2 U/ml). A novel bacterial isolate (Alcaligenes ammonioxydans) that generates chitinase was identified for the first time in Egypt, and it is regarded as one of the most productive isolates in this study. | ||||
| Keywords | ||||
| Alcaligenes spp; Chitinase; A. ammonioxydans; 16S rRNA; Shrimp shells; Egypt | ||||
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