Detection of ica C gene involved in biofilm formation in methicillin-resistant Staphylococcus aureus (MRSA) isolates
Abdelmoneim, A., Ali, R., Abdelhafeez, A., Khallaf, A., Molham, F., Tohamy Abdel-Aziz, M. (2023). Detection of ica C gene involved in biofilm formation in methicillin-resistant Staphylococcus aureus (MRSA) isolates. EKB Journal Management System, 4(4), 63-77. doi: 10.21608/ejmr.2023.218352.1408
Abeer Ahmed Abdelmoneim; Rasha Abdelaty Ali; Ahmed Hany Abdelhafeez; Ahmed Mahmoud Khallaf; Fatma Mohamed Molham; Mervat Abdel-Baseer Tohamy Abdel-Aziz. "Detection of ica C gene involved in biofilm formation in methicillin-resistant Staphylococcus aureus (MRSA) isolates". EKB Journal Management System, 4, 4, 2023, 63-77. doi: 10.21608/ejmr.2023.218352.1408
Abdelmoneim, A., Ali, R., Abdelhafeez, A., Khallaf, A., Molham, F., Tohamy Abdel-Aziz, M. (2023). 'Detection of ica C gene involved in biofilm formation in methicillin-resistant Staphylococcus aureus (MRSA) isolates', EKB Journal Management System, 4(4), pp. 63-77. doi: 10.21608/ejmr.2023.218352.1408
Abdelmoneim, A., Ali, R., Abdelhafeez, A., Khallaf, A., Molham, F., Tohamy Abdel-Aziz, M. Detection of ica C gene involved in biofilm formation in methicillin-resistant Staphylococcus aureus (MRSA) isolates. EKB Journal Management System, 2023; 4(4): 63-77. doi: 10.21608/ejmr.2023.218352.1408

Detection of ica C gene involved in biofilm formation in methicillin-resistant Staphylococcus aureus (MRSA) isolates

Egyptian Journal of Medical Research
Volume 4, Issue 4, October 2023, Page 63-77  XML PDF (899.31 K)
Document Type: Original Article
DOI: 10.21608/ejmr.2023.218352.1408
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Authors
Abeer Ahmed Abdelmoneim1; Rasha Abdelaty Ali email 2; Ahmed Hany Abdelhafeez3; Ahmed Mahmoud Khallaf4; Fatma Mohamed Molham5; Mervat Abdel-Baseer Tohamy Abdel-Aziz1
1Medical Microbiology and Immunology department, Faculty of Medicine, Beni-Suef University, Egypt
2Medical Microbiology and Immunology Department, Faculty of Medicine, Beni-Suef University, Egypt.
3M.B.B.CH Faculty of Medicine, Cairo University, Egypt
4Internal medicine department, Faculty of Medicine, Beni-Suef University, Egypt
5Medical Microbiology Department, Faculty of Pharmacy, Beni-Suef University, Egypt.
Abstract
Background: Staphylococcus aureus (S.aureus) is one of the gram-positive bacteria that causes a wide range of nosocomial infections. Our present study aimed to investigate genotypic and phenotypic aspects involved in biofilm formation in methicillin-resistant S.aureus strains isolated from hospital-acquired infections at Beni-Suef University Hospital Methodology: A total of 100 patients, 86 S. aureus strains were collected from all departments of Beni Suef University Hospital. The   antibiotic resistance pattern, phenotypes of biofilm formation and ica C gene were studied using Congo Red Agar (CRA) then molecular conformation was done by PCR. Results: We found that 80 out of 86 samples (93%) were mecicillin resistant S. aureus (MRSA). The highest frequency of resistance was found for oxacillin (95.3%), cefoxitin (88.4%), and ceftazidime (82.6%). Phenotypic results showed that 68.8% were high biofilm producers, while 10% and 21.2% were intermediate and low biofilm producers, respectively. From the 63 strong and intermediate biofilm producers isolates, we found that 55 specimens had the icaC gene (87.3%). Conclusion: Our study concluded that adherence ability and biofilm production are important for enhancing virulence factors among isolates of S. aureus strains.
Keywords
Staphylococcus aureus; MRSA; biofilm; ica gene; antibiotic resistance
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