Detection of Tuberculosis in slaughtered food animals by using recent technique | ||||
| Benha Veterinary Medical Journal | ||||
| Article 17, Volume 28, Issue 2, June 2015, Page 129-134 PDF (342.85 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2015.32488 | ||||
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| Authors | ||||
| Reham A. Amin1; Essam A. Nasr2; Ahmed M. El-Gaml3; El-Saeed M. Saafan4 | ||||
| 1Department of Food Control, Faculty of Veterinary Medicine, Benha University | ||||
| 2Veterinary Serum and Vaccine Research Institute, Abbasia | ||||
| 3Department of Bacteriology, Animal Health Research Institute, Mansoura | ||||
| 4Department of Meat hygiene, Animal Health Research Institute, Mansoura | ||||
| Abstract | ||||
| Bovine tuberculosis is still a major infectious disease among animal population and its transmission to human constitutes a public health problem. This study aimed to application of recent technique such as PCR for detection of tuberculosis among cattle and buffaloes carcasses. A grand total of 52 animals (40 cattle and 12 buffaloes) were routinely examined in the slaughterhouses of Dakahlia governorate, Egypt during the year 2014 for detection of tuberculosis. The suspected tuberculous lesions collected from cattle were 19 from respiratory, 8 from digestive, 12 from head and 1 generalized lesion. While in buffaloes, the tuberculous lesions were 3 from respiratory, 5 from digestive and 4 from head lesions. The bacteriological examination revealed that the isolation rate of Mycobacterium bovis (M.bovis) was 75% and 58.3% in cattle and buffaloes, respectively. The PCR technique was applied to confirm the results of bacteriological examination on 10 isolates of different mycobacteria and revealed 6 M.bovis isolates were positive with high sensitivity and specificity (100%) | ||||
| Keywords | ||||
| Bovine tuberculosis; PCR; Cattle Carcass; mycobacterium bovis | ||||
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