Detection of salmonella enteritidis in some meat products by using PCR | ||||
| Benha Veterinary Medical Journal | ||||
| Article 27, Volume 28, Issue 2, June 2015, Page 202-207 PDF (311.41 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2015.32503 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Ashraf A. Abd El-Tawab1; Fatma I. El-Hofy1; Khalid I. Alekhnawy2; Doaa M. Sharaf2 | ||||
| 1Bacteriology, Immunology and Mycology Dep., Fac .Vet .Med. Medicine ,Benha Uni. | ||||
| 2Animal Health Research Institute, Tanta branch | ||||
| Abstract | ||||
| A total of 150 random samples were collected from different supermarkets and retailers of different sanitation levels at Gharbeia governorate, Egypt as follows 70 samples of minced meat, 40 samples of sausage and 40 samples of beef burger. The aforementioned samples were subjected to bacteriological and serological applications to assess the prevalence of S. Enteritidis. The obtained results revealed that the incidence of S. Enteritidis in the examined samples of minced meat, sausage and beef burger were 1/70 (1.4%), 1/40(2.5%) and 0/40(0%) respectively. The isolates were submitted for serological analysis and revealed that Salmonella Enteritidis O 1,9,12 ad monophasic H:g, m. The antibiogram sensitivity test was applied upon the two isolates of S. Enteritidis and revealed that they are sensitive to chloramphinicol, amoxicillin, levofloxacin, ciprofloxacin, enrofloxacin and gentamycin but they were resistant to oxytetracycline. The virulence genes of S. Enteritidis isolates were determined by using multiple PCR technique for the two serologically detected Salmonella Enteritidis by using the following genes inv A, sef A, sop B and bcf C at 284bp, 310bp, 517bp, 467bp, respectively. | ||||
| Keywords | ||||
| S. Entritidis; meat products; simplex PCR and virulence genes | ||||
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