Rapid methods of diagnosis of Clostridium in broilers in Sharkia governorate
Abd El Tawab, A., Ammar, A., Elshorbagy, M., Mostafa, E. (2015). Rapid methods of diagnosis of Clostridium in broilers in Sharkia governorate. EKB Journal Management System, 28(2), 214-223. doi: 10.21608/bvmj.2015.32505
Ashraf A. Abd El Tawab; Ahmed M. Ammar; Mamdouh A. Elshorbagy; Ehab W. Mostafa. "Rapid methods of diagnosis of Clostridium in broilers in Sharkia governorate". EKB Journal Management System, 28, 2, 2015, 214-223. doi: 10.21608/bvmj.2015.32505
Abd El Tawab, A., Ammar, A., Elshorbagy, M., Mostafa, E. (2015). 'Rapid methods of diagnosis of Clostridium in broilers in Sharkia governorate', EKB Journal Management System, 28(2), pp. 214-223. doi: 10.21608/bvmj.2015.32505
Abd El Tawab, A., Ammar, A., Elshorbagy, M., Mostafa, E. Rapid methods of diagnosis of Clostridium in broilers in Sharkia governorate. EKB Journal Management System, 2015; 28(2): 214-223. doi: 10.21608/bvmj.2015.32505

Rapid methods of diagnosis of Clostridium in broilers in Sharkia governorate

Benha Veterinary Medical Journal
Article 29, Volume 28, Issue 2, June 2015, Page 214-223  XML PDF (629.3 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2015.32505
View on SCiNiTO View on SCiNiTO
Authors
Ashraf A. Abd El Tawab1; Ahmed M. Ammar2; Mamdouh A. Elshorbagy3; Ehab W. Mostafa4
1Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Benha University
2Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Zagazig University.
3Animal Health Research Institute, Dokki
4Veterinary Medicine Directorate Sharkia
Abstract
A total 100 samples of liver and intestine from broilers (apparently diseased) from 13 poultry farms
were analyzed to detect the incidence of C. perfringes. The samples were examined bacteriologically by
cultivating on CMC and blood agar, typical colonies were identified and biochemically confirmed. The
total number of positive samples was 46 (46%). C. perfringens showed very high sensitivity to
enrofloxacin, high sensitivity to amoxicillin and cefoperazone, but resistant for mupirocin and
azteronam. By making artificial infection for mice, the mice died after 24 hours and by examination
histopathologicaly, it showed liver degeneration and desquamation of epithelial cells of intestine.
Multiplex polymerase chain reaction method (PCR) was amplified for toxin genotyping by using primer
for clostridium perfringes alpha toxin which amplified at 402 bp
Keywords
Clostridium perfringes; broiler; Sharkia; Liver; Intestine
Statistics
Article View: 102
PDF Download: 253