Biochemical characterization and in vitro cytotoxic activity of the Egyptian scorpion Leiurus quinquestriatus whole body extract
Salama, W., El-Naggar, S., Elnewehy, A., El desoky, N. (2023). Biochemical characterization and in vitro cytotoxic activity of the Egyptian scorpion Leiurus quinquestriatus whole body extract. EKB Journal Management System, 7(3), 57-63. doi: 10.21608/jcbr.2023.199517.1295
Wesam Salama; Sabry A. El-Naggar; Abdelwahab Elnewehy; Nabila Ibrahim El desoky. "Biochemical characterization and in vitro cytotoxic activity of the Egyptian scorpion Leiurus quinquestriatus whole body extract". EKB Journal Management System, 7, 3, 2023, 57-63. doi: 10.21608/jcbr.2023.199517.1295
Salama, W., El-Naggar, S., Elnewehy, A., El desoky, N. (2023). 'Biochemical characterization and in vitro cytotoxic activity of the Egyptian scorpion Leiurus quinquestriatus whole body extract', EKB Journal Management System, 7(3), pp. 57-63. doi: 10.21608/jcbr.2023.199517.1295
Salama, W., El-Naggar, S., Elnewehy, A., El desoky, N. Biochemical characterization and in vitro cytotoxic activity of the Egyptian scorpion Leiurus quinquestriatus whole body extract. EKB Journal Management System, 2023; 7(3): 57-63. doi: 10.21608/jcbr.2023.199517.1295

Biochemical characterization and in vitro cytotoxic activity of the Egyptian scorpion Leiurus quinquestriatus whole body extract

Egyptian Journal of Cancer and Biomedical Research
Volume 7, Issue 3, September 2023, Page 57-63  XML PDF (1.71 MB)
Document Type: Original Article
DOI: 10.21608/jcbr.2023.199517.1295
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Authors
Wesam Salama email 1; Sabry A. El-Naggarorcid 2; Abdelwahab Elnewehy3; Nabila Ibrahim El desoky4
1Zoology Department, Faculty of Science, Tanta University
2Zoology Department, Faculty of Science, Tanta University, Tanta 3111, Egypt
3Faculty of Science, Tanta University
4Histology and Histochemistry Division, Zoology Department, Faculty of Science, Tanta University, Egypt
Abstract
Invertebrates are considered potential sources for drug development. This study was evaluated the biochemical characterization and the in vitro cytotoxic effect of Leiurus quinquestriatus whole-body extract (LQWBE) against HepG-2 and MCF-7 cell lines. For characterization, the total proteins (T.P), lipids (T.L), carbohydrates (T.C), sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), gas chromatography-mass spectroscopy (GC-MS), and minerals contents were evaluated in LQWB. The results showed that the contents of T.P, T.L, and T.C were 712 ± 8.5 mg/g, 68 ± 3.8 mg/g, and 216 ± 5.2, respectively. SDS-PAGE analysis showed that LQWBE has three bands of 12.5, 20, and 30 kDa. In addition, GC-MS analysis revealed that the presence of 9, 12-Octadecadienoic acid (Z, Z)-, methyl ester at the highest peak area. Sodium, potassium, magnesium, and calcium levels were 9.1 ± 0.16, 35 ± 0.28, 0.2± 0.01, and 5.5 ± 0.77 mg/g extract, respectively. LQWBE showed a mild cytotoxic activity in vitro against HepG-2 and MCF-7 human cancer cells. The LQWBE IC50 against HepG-2 and MCF-7 cell lines were 992 µg/ml ± 49.2 and more than 1000µg/ml after 24 hours of treatment.
Keywords
GC-MS; Cytotoxicity; In vitro; Leiurus quinquestratus Scorpion; cancer cell lines
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