Effects of Date Palm Fruit (Phoenix Dactylifera L.) as a Dietary Additive on Some Physiological Parameters and Radiographic Bone Density in Heat Stressed Male Rats | ||||
| Journal of Applied Veterinary Sciences | ||||
| Article 5, Volume 9, Issue 1, January 2024, Page 42-54 PDF (724.54 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/javs.2023.239423.1279 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Marian M. Eskander 1; Salma I. El Samannoudy1; Aya kh. Hendawy2; Elham A. Hassan 3; Mahmoud Z. Attia  4
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| 1Department of Physiology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt | ||||
| 2Department of Physiology, Faculty of Veterinary Medicine, Cairo University, Giza , Egypt. | ||||
| 3Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Cairo University, Egypt | ||||
| 4Department of Physiology, Faculty of Veterinary Medicine, Cairo University, Egypt | ||||
| Abstract | ||||
| Heat stress is Heat stress is a life-threatening condition with a detrimental impact on the physiological functions of both humans and animals. The present study aimed to investigate the effect of date palm fruit on physiological functions and bone density and to document the protective effect of date palm in mitigating the negative impact of heat stress in rats. Thirty-two mature male Sprague Dawley rats weighing 170–200 g were randomly divided into four groups (8 rats/group): group 1 (control) was provided with standard diet pellets; group 2 (heat stress) rats received a standard diet and were exposed to artificial heat stress (43°C for 60 min/day); group 3 (date palm fruit) rats were given date palm fruit at a dose of 1 g/kg body weight; and group 4 (date palm fruit + heat stress) rats received date palm fruit with the same dose and were exposed to the same protocol of heat stress. Diet protocols started from the beginning of the experiment and continued till the end of the study at 2 months. Heat stress was induced (groups 2 and 4) daily during the second month of the experiment. Hematobiochemical and oxidative stress parameters, histopathological examination of the liver, kidney, and adrenal gland, and quantitative evaluation of radiographic bone density were evaluated at the end of the study. Results demonstrated heat stress resulted in significantly increased leucocyte count, decreased RBCs, platelet count, and Hb concentration (P< 0.01). Significant increases in ALT, AST, ALP, urea and creatinine levels (P < 0.01) with concurrent histopathological changes in the liver and kidney were also recorded in heat-stressed rats. Oxidative stress biomarkers, glucocorticoids, were increased with heat stress (P< 0.01). Serum calcium level and radiographic bone density were significantly decreased in the heat stress group. Rats supplemented with date palm and subjected to heat stress exhibited an insignificant change in physiologic parameters (P> 0.05) compared to control rats. In conclusion, date palm fruit demonstrated a protective effect against the deleterious changes of heat stress in rats by maintaining physiological parameters and improving bone turnover. Dietary supplementation with date palm prior to exposure to heat stress is safe and effective in protecting against the life-threatening adverse effects of heat stress. | ||||
| Keywords | ||||
| Bone; Date fruit; Heat-stressed; kidney; Liver; Oxidative stress | ||||
| References | ||||
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ABDEEN, A., SAMIR, A., ELKOMY, A., ABOUBAKER, M., HABOTTA, O. A., GABER, A., ALSANIE, W. F., ABDULLAH, O., ELNOURY, H. A., BAIOUMY, B., IBRAHIM, S. F., and ABDELKADER, A., 2021. The potential antioxidant bioactivity of date palm fruit against gentamicin-mediated hepato-renal injury in male albino rats. Biomedicine and Pharmacotherapy, 143: 112154. https://doi.org/10.1016/j.biopha.2021.112154
ABDELAZIZ, D.H.A., and ALI, S.A., 2014. The protective effect of Phoenix dactylifera L. seeds against CCl4-induced hepatotoxicity in rats. Journal of Ethno pharmacology, 155(1): 736–743. https://doi.org/10.1016/j.jep.2014.06.026
ADERAO, G. N., SAHOO, A., KUMAWAT, P. K., and BHATT, R. S., 2020. Effect of complete feed block with tree leaves rich in hydrolysable and condensed tannins on nutrient utilization, rumen fermentation and growth performance of lambs. Journal of Animal Physiology and Animal Nutrition, 104(1): 101–108. https://doi.org/10.1111/jpn.13261
AHMED, M.B., HASONA, N.A.S., and SELEMAIN, H.A.H., 2008. Protective effects of extract from dates (Phoenix dactylifera L.) and ascorbic acid on thioacetamide-induced hepatotoxicity in rats. 7 (3): 193-201. https://doi.org/10.22037/ijpr.2010.765.
AL-FARSI, M.A., and LEE, C.Y., 2008a. Nutritional and functional properties of dates: A review. In Critical Reviews in Food Science and Nutrition, 48(10): 877–887. https://doi.org/10.1080/10408390701724264
AL-FARSI, M.A., and LEE, C.Y., 2008b. Optimization of phenolics and dietary fibre extraction from date seeds. FoodChemistry,108(3): 977–985. https://doi.org/10.1016/j.foodchem.2007.12.009
ALHIDARY, I. A., SHINI, S., AL JASSIM, R.A.M., and GAUGHAN, J.B., 2012. Physiological responses of australian merino wethers exposed to high heat load. Journal of Animal Science, 90(1):212–220. https://doi.org/10.2527/jas.2011-3972
ALLAITH, A.A.A. 2008. Antioxidant activity of Bahraini date palm (Phoenix dactylifera L.) fruit of various cultivars. International Journal of Food Science and Technology, 43(6): 1033–1040. https://doi.org/10.1111/j.1365-2621.2007.01558.x
AL-MSSALLEM, M.Q. 2020. The role of date palm fruit in improving human health. Journal of Clinical and Diagnostic Research, 14: 1-6. https://doi.org/10.7860/JCDR/2020/43026.13442
ASMIS, R., WANG, Y., XU, L., KISGATI, M., BEGLEY, J. G., and MIEYAL, J. J., 2005. A novel thiol oxidation‐based mechanism for adriamycin‐induced cell injury in human macrophages. The Federation of American Societies for Experimental Biology Journal, 19(13):1866–1868. https://doi.org/10.1096/fj.04-2991fje
AYDIN, S., 2015. A short history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA. Peptides, 72: 4–15. Doi: 10.1016/j.peptides.2015.04.012
BASHANDY, M.A., ABDEL-RASHEID, H.G., and GAD, H.K., 2018. Possible effect of date palm fruit extract on some biochemical and haematological parameters in rats intoxicated with methomylinsecticide. In Al Azhar Bulletin of Science, 29(1):35-46.
BAUMGARD, L.H., and RHOADS, R.P., 2013. Effects of heat stress on post absorptive metabolism and energetics. Annual Review of Animal Biosciences, 1: 311–337. https://doi.org/10.1146/annurev-animal-031412-103644
BENMEDDOUR, Z., MEHINAGIC, E., MEURLAY, D. LE, and LOUAILECHE, H., 2013. Phenolic composition and antioxidant capacities of ten Algerian date (Phoenix dactylifera L.) cultivars: A comparative study. Journal of Functional Foods, 5(1): 346–354. https://doi.org/10.1016/j.jff.2012.11.005
BERGMEYER, H.U., HORDEN, M., and REJ, R., 1986. Approved recommendation on international federation of clinical chemistry (IFCC) methods for the measurement of catalytic concentration of enzymes alanine aminotransferase. Clinical Chemistry and Clinical Biochemistry, 24(7): 481-495.
BHUSARI, S., HEARNE, L. B., SPIERS, D. E., LAMBERSON, W. R., and ANTONIOU, E., 2008. Transcriptional profiling of mouse liver in response to chronic heat stress. Journal of Thermal Biology, 33(3):157–167. https://doi.org/10.1016/j.jtherbio.2008.01.001
CASTRO, B. B. A. DE, CARMO, W. B., OLIVEIRA, R. S. M. F., PETERS, V. M., JORGETTI, V., CUSTODIO, M. R., and SANDERS-PINHEIRO, H., 2020. Digital radiography as an alternative method in the evaluation of bone density in uremic rats. Jornal Brasileiro de Nefrologia, 42(1): 8–17. https://doi.org/10.1590/2175-8239-jbn-2019-0008
DAPSON, R.W., and HOROBIN, R.W., 2009. Dyes from a twenty-first century perspective. Biotechnic & Histochemistry, 84(4): 135-137. doi:10.1080/10520290902908802
DE KLERK, G., VAN DER VELDE, D., VAN DER PALEN, J., VAN BERGEIJK, L., and HEGEMAN, J.H., 2009. The usefulness of dual energy X-ray and laser absorptiometry of the calcaneus versus dual energy X-ray absorptiometry of hip and spine in diagnosing manifest osteoporosis. Archives of Orthopaedic and Trauma Surgery, 129(2): 251–257.https://doi.org/10.1007/s00402-008-0755-y
DESOKY, A.A., and KAMEL, N.N., 2018. Growth performance and blood constituents of brown and white japanese quail reared under heat stress conditions. Egyptian Poultry Science Journal, 38(3): 723-733.
DOUMAS, B. T., WATSON, W. A., and BIGGS, H. G., 1971. Albumin standards and the measurement of serum albumin with bromocresol green. Clinica Chimica Acta, 31:87-96.
EL FOUHIL, A.F., AHMED, A.M., ATTEYA, M., MOHAMED, R.A., MOUSTAFA, A.S., DARWISH, H.H., FATHALLA, A., and El FOUHIL, I., 2013. An extract from date seeds stimulates endogenous insulin secretion in streptozotocin-induced type I diabetic rats. Functional Foods in Health and Disease, 3(11):441.
FATHY, A. H., BASHANDY, M. A., BASHANDY, S. A. E., MANSOUR, A. M., and AZAB, K.S., 2018. The beneficial effect of natural antioxidants from olive oil with fig and date palm fruit extracts on biochemical and hematological parameters in rats treated with doxorubicin and γ-radiation. Facets, 3(1):722–735. https://doi.org/10.1139/facets-2017-0080
FERNÁNDEZ-LÓPEZ, J., VIUDA-MARTOS, M., SAYAS-BARBERÁ, E., NAVARRO-RODRÍGUEZ DE VERA, C. and PÉREZ-ÁLVAREZ, J.Á., 2022. Biological, nutritive, functional and healthy potential of date palm fruit (Phoenix dactylifera L.): Current research and future prospects. Agronomy, 12(4): 876.
GARNETT, T., CONN, V., and KAISER, B. N., 2009. Root based approaches to improving nitrogen use efficiency in plants. Plant, Cell and Environment, 32(9): 1272–1283. https://doi.org/10.1111/j.1365-3040.2009.02011.x
GHNIMI, S., UMER, S., KARIM, A., and KAMAL-ELDIN, A., 2017. Date fruit (Phoenix dactylifera L.): An underutilized food seeking industrial valorization. NFS Journal, 6: 1–10. https://doi.org/10.1016/j.nfs.2016.12.001
HALDER, S., SARKARA, M., DEYA, S., GHOSHD, P., BHUNIA, S.K., BANDYOPADHYAY, D. and GIRI, B., 2020. Melatonin amelioratesheat stress induced dysregulation of testicular function in Wistar rat by restoring tissue health, hormone and antioxidant status and modulating heat shock protein expression. International Journal of Life Science and Pharma Research, 10(5): L31-43. https://doi.org/10.22376/ijpbs/lpr.2020.10.5.l31-43
HAQUE, N., LUDRI, A., HOSSAIN, S.A. and ASHUTOSH, M., 2013. Impact on hematological parameters in young and adult Murrah buffaloes exposed to acute heat stress. Buffalo Bulletin, 32(4):321-326 https://doi.org/10.14456/ku-bufbu.2013.44
HASHISH, E.A., and ELGAML, S.A., 2016. Role of nicotinic acid in mitigating methomyl induced acute toxicity in albino ratsJournal of Clinical & Experimental Pathology, 6(268): 2161-0681. https://doi.org/10.4172/2161-0681.1000268
HUDDLESTON, A., 2012. Quantitative methods in bone densitometry. Springer Science & Business Media.1st ed. Springer New York, NY https://doi.org/10.1007/978-1-4613-1725-8
HUSSAIN MALLHI, T., QADIR, M.I., ALI, M., AHMAD, B., and KHAN, Y.H., 2014. Ajwa date (Phoenix dactylifera): an emerging plant in pharmacological research. Pakistan journal of Pharmaceutical Sciences, 27(3): 607-616.
HUSSAIN, M.I., FAROOQ, M. and SYED, Q.A., 2020. Nutritional and biological characteristics of the date palm fruit (Phoenix dactylifera L.): A review. Food Bioscience, 34: 100509. https://doi.org/10.1016/j.fbio.2019.100509
JAGETIA, G. C., GANAPATHI, N. G., VENKATESH, P., RAO, N., and BALIGA, M. S., 2006. Evaluation of the radioprotective effect of Liv 52 in mice. Environmental and Molecular Mutagenesis, 47(7): 490–502. https://doi.org/10.1002/em.20218
KHALID, S., KHALID, N., KHAN, R. S., AHMED, H., and AHMAD, A., 2017. A review on chemistry and pharmacology of Ajwa date fruit and pit. Trends in Food Science and Technology, 63: 60–69. https://doi.org/10.1016/j.tifs.2017.02.009
KIM, G. H., KIM, J. E., RHIE, S. J., and YOON, S., 2015. The Role of Oxidative Stress in Neurodegenerative Diseases. Experimental Neurobiology, 24(4): 325–340. https://doi.org/10.5607/en.2015.24.4.325
KINDS, M.B., BARTELS, L.W., MARIJNISSEN, A.C.A., VINCKEN, K. L., VIERGEVER, M. A., LAFEBER, F.P.J.G., and DE JONG, H W. A.M., 2011. Feasibility of bone density evaluation using plain digital radiography. Osteoarthritis and Cartilage, 19(11): 1343–1348. https://doi.org/10.1016/j.joca.2011.08.003
KINN RØD, A.M., HARKESTAD, N., JELLESTAD, F.K., and MURISON, R., 2017. Comparison of commercial ELISA assays for quantification of corticosterone in serum. Scientific Reports, 7(1): 6748. https://doi.org/10.1038/s41598-017-06006-4
KORACEVIC, D., KORACEVIC, G., DJORDJEVIC, V., ANDREJEVIC, S., and COSIC, V., 2001. Method for the measurement of antioxidant activity in human fluids. Journal of Clinical Pathology, 54(5): 356–361. https://doi.org/10.1136/jcp.54.5.356
KUMBHAR, S., KHAN, A. Z., PARVEEN, F., NIZAMANI, Z. A., SIYAL, F. A., EL-HACK, M. E. A., GAN, F., LIU, Y., HAMID, M., NIDO, S. A., and HUANG, K., 2018. Impacts of selenium and vitamin E supplementation on mRNA of heat shock proteins, selenoproteins and antioxidants in broilers exposed to high temperature. AMB Express, 8(1): 112. https://doi.org/10.1186/s13568-018-0641-0
LONGANBACH, S., CHAPMAN, D., BOURLIER WALDRON, K., and MIERS, M., 2007. Automated cell counting instrumentation and point of care testing. Hematology: Clinical Principles and Applications 3rd edn. Saunders, Elsevier Inc., Saint Louis, MO.
LUKERT, B.P., and RAISZ, L.G., 1990. Glucocorticoid-induced osteoporosis: pathogenesis and management. Annals of Internal Medicine, 112(5): 352-364.
MALYAR, R.M., TANHA, J., ZIAUDDIN, Z., ISMAIL, K.S., IBRAHIMI, M., KHALID, A.U.R., HANIFULLAH, B., BANUREE, S.A.H., and VISTRO, W.A., 2021. Amelioration of heat stress-induced hepatic injury in wistar rats by zinc-enriched probiotics: Role of hepatic antioxidant status and serum enzymes activity. Pure and Applied Biology, 10(4): 1494-1503. https://doi.org/10.19045/bspab.2021.100155
MICHAEL, H. N., SALIB, J. Y., and ESKANDER, E. F., 2013. Bioactivity of diosmetin glycosides isolated from the epicarp of date fruits, Phoenix dactylifera, on the biochemical profile of alloxan diabetic male rats. Phytotherapy Research, 27(5):699–704. https://doi.org/10.1002/ptr.4777
MOORTHI, R. N., and MOE, S. M., 2013. Recent advances in the noninvasive diagnosis of renal osteodystrophy. Kidney International 84(5):886–894.. https://doi.org/10.1038/ki.2013.254
NAJAFIAN, L., and BABJI, A. S., 2012. A review of fish-derived antioxidant and antimicrobial peptides: Their production, assessment, and applications. Peptides, 33(1): 178–185. https://doi.org/10.1016/j.peptides.2011.11.013
OHKAWA, H., OHISHI, N., and YAGI, K., 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95(2):351-358.
PARAMITHA, A.K. and MACHFUDLO, H., 2020. Palm Date Increase Adolescents Hemoglobin Levels.Earth and Environmental Science, 519(1): IOP Publishing.https://doi.org/10.1088/1755-1315/519/1/012032
RENAUDEAU, D., COLLIN, A., YAHAV, S., DE BASILIO, V., GOURDINE, J. L., and COLLIER, R. J., 2012. Adaptation to hot climate and strategies to alleviate heat stress in livestock production. Animal, 6(5):707–728. https://doi.org/10.1017/S1751731111002448
RISTELI, J., WINTER, W.E., KLEEREKOPER, M., and RISTELI, L., 2015. Disorders of bone and mineral metabolism. Tietz Fundamentalis of Clinical Chemistry and Molecular Diagnostics. 7th ed. Bruns: Saunders, pp.741-68.
SARIS, N.E. 1987. Revised international federation of clinical chemistry (IFCC) method for aspartate aminotransferase. Clinical Chemistry, 24: 720-721.
SINGH, V., GUIZANI, N., ESSA, HAKKIM, and RAHMAN, F. L., 2012. Comparative analysis of total phenolics, flavonoid content and antioxidant profile of different date varieties (Phoenix dactylifera L.) from Sultanate of Oman. International Food Research Journal, 19 (3): 1063-1070.
SPENCER, K., and PRICE, C.P., 1980. A review of non enzyme mediated reactions and their applications to centrifugal analysers. Centrifugal Analysers in Clinical Chemistry. Eastbourne: Praeger, pp.231-253.
TIAN, X.Z., LI, J.X., LUO, Q.Y., WANG, X., XIAO, M.M., ZHOU, D., LU, Q. and CHEN, X., 2022. Effect of supplementation with selenium-yeast on muscle antioxidant activity, meat quality, fatty acids and amino acids in goats. Frontiers in Veterinary Science, 8: 813672. https://doi.org/10.3389/fvets.2021.813672
TIETZ, N. W., RINKER, A. D., and SHAW, L. M., 1983. International federation of clinical chemistry (IFCC) methods for the measurement of catalytic concentration of enzymes alkaline phosphatase. Journal of Clinical Chemistry and Clinical Biochemistry, 21:731-748.
TRINDER. P. 1969. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Annals of Clinical Biochemistry, 6: 24-28.
WEICHSELBAUM, T.E., 1946. An accurate and rapid method for the determination of proteins in small amounts of blood serum and plasma. American Journal of Clinical Pathology, 10: 40.
YUN, J.H., TOMAS-BARBERAN, F.A., KADER, A.A., and MITCHELL, A. E., 2006. The flavonoid glycosides and procyanidin composition of Deglet Noor dates (Phoenix dactylifera). Journal of Agricultural and Food Chemistry, 54(6): 2405–2411. https://doi.org/10.1021/jf0581776
ZAAKOUK, S., EL-RASHEID, A., HESHAM, G., BELAL, A. and ELFEKY, K., 2018. Effect of balanites aegyptiaca (heglig dates) and persea americana (avocado fruit) on some hematological and biochemical parameters in streptozotocin induced diabetic male rats. Al-Azhar Bulletin of Science, 29(2-C):49-59. https://doi.org/10.21608/absb.2018.33818
ZHENG, Y., DAI, W., HU, X., and HONG, Z., 2020. Effects of dietary glycine selenium nanoparticles on loin quality, tissue selenium retention, and serum antioxidation in finishing pigs. Animal Feed Science and Technology, 260: 114345 https://doi.org/10.1016/j.anifeedsci.2019.114345
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