Green Synthesis of Copper Nanoparticles Using Coffee Arabica: Larvicidal and Biochemical Study
Abdulwahab, Y., Ahmad, A., Wahid, I., Taba, P. (2023). Green Synthesis of Copper Nanoparticles Using Coffee Arabica: Larvicidal and Biochemical Study. EKB Journal Management System, 9(4), 199-211. doi: 10.21608/jbaar.2023.326117
Yousef Abdulwahab; Ahyar Ahmad; Isra Wahid; Paulina Taba. "Green Synthesis of Copper Nanoparticles Using Coffee Arabica: Larvicidal and Biochemical Study". EKB Journal Management System, 9, 4, 2023, 199-211. doi: 10.21608/jbaar.2023.326117
Abdulwahab, Y., Ahmad, A., Wahid, I., Taba, P. (2023). 'Green Synthesis of Copper Nanoparticles Using Coffee Arabica: Larvicidal and Biochemical Study', EKB Journal Management System, 9(4), pp. 199-211. doi: 10.21608/jbaar.2023.326117
Abdulwahab, Y., Ahmad, A., Wahid, I., Taba, P. Green Synthesis of Copper Nanoparticles Using Coffee Arabica: Larvicidal and Biochemical Study. EKB Journal Management System, 2023; 9(4): 199-211. doi: 10.21608/jbaar.2023.326117

Green Synthesis of Copper Nanoparticles Using Coffee Arabica: Larvicidal and Biochemical Study

Journal of Bioscience and Applied Research
Volume 9, Issue 4, December 2023, Page 199-211  XML PDF (1.37 MB)
Document Type: Original Article
DOI: 10.21608/jbaar.2023.326117
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Authors
Yousef Abdulwahab1; Ahyar Ahmad email 2; Isra Wahid3; Paulina Taba2
1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar, Indonesia./Department of Biology, Faculty of Applied Sciences, Taiz University, Taiz, Yemen.
2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar, Indonesia
3Department of Parasitology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
Abstract
Aedes aegypti is a major problem in transmitting dangerous diseases to humans, including dengue fever. Therefore, this study aimed to synthesize copper nanoparticles from Coffee arabica and use them in the control of this dangerous insect. To confirm and characterize the formation of copper nanoparticles ultraviolet spectrophotometer, Fourier transform infrared spectrometry, and X-ray diffraction methods were used. To study the toxicity of copper nanoparticles on some biochemical parameters in the body of the insect, the amount of glucose, total protein, and metabolic enzymes such as aspartate transferase (AST), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) were analyzed. The results indicated that the highest mortality rate in Aedes aegypti larva was 96.3 % at a concentration of 100 ppm, and LC50 and LC90 were 5.7 and 36. 5%, respectively. It also showed that the lowest rate of emergence of pupae is 3.75 % at a concentration of 100 ppm, and the lowest rate of emergence of an adult insect is 2.5 % at a concentration of 50 ppm. Biochemical analyses indicate a significant decrease in the amount of total protein, alkaline phosphatase, and lactate dehydrogenase enzyme, but a significant increase in the amount of glucose and aspartate transferase enzyme was observed. Based on the obtained results, copper nanoparticles are considered a strong and promising candidate for controlling Aedes aegypti mosquito larvae and inhibiting their developmental stages.
Keywords
Aedes aegypti; copper nanoparticles; Coffee arabica; larvicidal; biochemical study
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