Keratinase Production from Chicken Feather by New Egyptian Local Streptomyces Isolates

Abd El Nasser, Nadia H.; Aly, Amal M.; Hanafy, Shimaa S.; Motawe, Fatma H.

doi:10.21608/ejm.2023.238612.1235

	Keratinase Production from Chicken Feather by New Egyptian Local Streptomyces Isolates
Egyptian Journal of Microbiology
Volume 58, Issue 1, 2023, Page 25-33 PDF (1.14 MB)
Document Type: Original Article
DOI: 10.21608/ejm.2023.238612.1235
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Authors
Nadia H. Abd El Nasser¹; Amal M. Aly¹; Shimaa S. Hanafy²; Fatma H. Motawe ¹
¹Microbial Chemistry Department, Biotechnology Research Institute , National Research Centre, 33 El Behouth st., Dokki P.O. 12622, Giza, Egypt
²Biochemistry Department , Biotechnology Research Institute , National Research Centre, 33 El Behouth st., Dokki P.O. 12622, Giza, Egypt
Abstract
The main objective of the current research was to isolate new Streptomyces species that has the ability to degrade chicken feathers through keratinase production. Nineteen Egyptian local Streptomyces strains were isolated from soil and tested for their ability to produce keratinase enzyme from chicken feathers. Maximum keratinolytic activity was achieved with a novel Streptomyces isolate strain no 6. that was identified by 16S rDNA method with a given name of Streptomyces sp. NRC FM (Accession no. LC456058). The optimum culture conditions for the highest keratinase production were found to be a pH 9.5, at a temperature of 37˚C and agitation speed of 180 rpm. Interestingly, chicken feathers were completely degraded after 9 days of incubation with the novel strain. Keratinase was precipitated using acetone and its molecular weight was estimated to be 30 and 42 kDa using SDS-PAGE. The results obtained suggest that the isolated novel Streptomyces sp., NRC FM, can be considered as a useful biotechnology tool for valorization of keratin-containing wastes.
Keywords
Feather keratins; Keratinase enzyme; Keratinase-producing microorganisms; Streptomyces sp


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