Bacteriological and molecular studies on staphylococcus aureus isolated from raw milk
AbdEL-Tawab, A., Abou El-Roos, N., El-Gendy, A. (2015). Bacteriological and molecular studies on staphylococcus aureus isolated from raw milk. EKB Journal Management System, 28(1), 88-97. doi: 10.21608/bvmj.2015.32658
Ashraf A. AbdEL-Tawab; Nahla A. Abou El-Roos; Asmaa A.M. El-Gendy. "Bacteriological and molecular studies on staphylococcus aureus isolated from raw milk". EKB Journal Management System, 28, 1, 2015, 88-97. doi: 10.21608/bvmj.2015.32658
AbdEL-Tawab, A., Abou El-Roos, N., El-Gendy, A. (2015). 'Bacteriological and molecular studies on staphylococcus aureus isolated from raw milk', EKB Journal Management System, 28(1), pp. 88-97. doi: 10.21608/bvmj.2015.32658
AbdEL-Tawab, A., Abou El-Roos, N., El-Gendy, A. Bacteriological and molecular studies on staphylococcus aureus isolated from raw milk. EKB Journal Management System, 2015; 28(1): 88-97. doi: 10.21608/bvmj.2015.32658

Bacteriological and molecular studies on staphylococcus aureus isolated from raw milk

Benha Veterinary Medical Journal
Article 10, Volume 28, Issue 1, March 2015, Page 88-97  XML PDF (359.94 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2015.32658
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Authors
Ashraf A. AbdEL-Tawab1; Nahla A. Abou El-Roos2; Asmaa A.M. El-Gendy2
1Bacteriology, Immunology and Mycology Dep., Fac. Vet. Med. BenhaUniv.
2Animal Health Research “Shebin El- Kom branch”
Abstract
The present study was performed on a total of 100 raw buffalo Milk samples from different sources at
El-Menofiya Governorate that the inspected samples were taken in ice box within an a hour for
bacteriological examination. The results revealed that 80 out of 100 samples (80%) were positive for
staphylococcus spp. Out of those 80 samples, 42(53%) were coagulase positive Staphylococci spp while
38(47%) were coagulase Negative Staphylococci spp. Out of those 42 coagulase positive Staphylococci
spp,31 were (73.8%) Staph. aureus. In vitro Gentamycin, Trimethoprim / sulphamethazole, Ampicillin,
Cephadrine were the most proper antibiotic against isolated Staph. aureus. By using PCR Spa virulence
gene was detected in two studied strains while, enterotoxin E was detected only in one strain out of 6
studied strains. Meanwhile, hIyA and enterotoxin (Sea, Seb, Sec, Sed) virulence genes were not detected
in all studied strains
Keywords
Staphylococcus aureus; Raw milk; PCR
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